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Human Beta2-Microglobulin ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD003R 96 wells (1 kit) $417,87
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the direct quantitative determination of β2 Microglobulin by enzyme immunoassay in human serum. For research use only.



Sample Requirements

20 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.2–10 mg/L

Limit of Detection

0.1 mg/L

Intra-assay (Within-Run)

CV = 4.9%

Inter-assay (Run-to-Run)

CV = 6.7%



  • RUO
  • calibration range 0.2-10 mg/L
  • limit of detection 0.1 mg/L
  • intra-assay CV = 4.9%
  • inter-assay CV = 6.7%

Research topic

Oncology, Renal disease


β2-microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β2-M is continuously secreted into the circulatory system and therefore maintains a balanced serum level. Clinical Trends: − Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M. − β2-M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis. − Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M. β2-M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2-M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.

Summary References (18)

References to Beta2-Microglobulin

  • Bhalla RB, Safai B, Mertelsmann R, Schwartz MK. Abnormally high concentrations of beta 2 microglobulin in acquired immunodeficiency syndrome (AIDS) patients. Clin Chem. 1983 Aug;29 (8):1560
  • Brodsky FM, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul;9 (7):536-45
  • Burnett RW. Accurate estimation of standard deviations for quantitative methods used in clinical chemistry. Clin Chem. 1975 Dec;21 (13):1935-8
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Domingo P, Martinez E, Martinez C, Fuster M, Cortes M, Sambeat MA, Ris J, Cadafalch J. Prognostic value of serum beta 2-microglobulin in HIV infection. Lancet. 1992 Aug 8;340 (8815):371-2
  • Evrin PE, Jansson NO, Bergdahl A. A turbidimetric immunochemical method for determination of serum beta 2-microglobulin using a centrifugal analyzer. Clin Chim Acta. 1986 Mar 16;155 (2):151-7
  • Fahey JL, Taylor JM, Detels R, Hofmann B, Melmed R, Nishanian P, Giorgi JV. The prognostic value of cellular and serologic markers in infection with human immunodeficiency virus type 1. N Engl J Med. 1990 Jan 18;322 (3):166-72
  • Francioli P, Clement F, Vaudois CH. Beta 2-microglobulin and immunodeficiency in a homosexual man. N Engl J Med. 1982 Nov 25;307 (22):1402-3
  • Lacey JN, Forbes MA, Waugh MA, Cooper EH, Hambling MH. Serum beta 2-microglobulin and human immunodeficiency virus infection. AIDS. 1987 Jul;1 (2):123-7
  • LeClair K, Van Agthoven A, Terhorst C. A rapid method for isolation of antigenically active human cell surface antigens associated with beta 2-microglobulin using a monoclonal antibody. J Immunol Methods. 1981;41 (2):137-44
  • Ling TG, Mattiasson B. A general study of the binding and separation in partition affinity ligand assay. Immunoassay of beta 2-microglobulin. J Immunol Methods. 1983 May 13;59 (3):327-37
  • Nilsson K, Evrin PE, Berggard I, Ponten J. Involvement of lymphoid and nonlymphoid cells in the production of beta2-microglobulin-a homologue of the constant domains of IgG. Nat New Biol. 1973 Jul 11;244 (132):44-5
  • O'Reilly D, Whicher J, Sanderson AR. A simple, precise and sensitive nephelometric assay for beta 2-microglobulin in body fluids. J Immunol Methods. 1983 Feb 25;57 (1-3):265-73
  • Sonnerborg AB, von Stedingk LV, Hansson LO, Strannegard OO. Elevated neopterin and beta 2-microglobulin levels in blood and cerebrospinal fluid occur early in HIV-1 infection. AIDS. 1989 May;3 (5):277-83
  • Tachibana K, Tanaka E, Usuda S, Okamoto H, Imai M, Nakamura T, Miyakawa Y, Mayumi M. A two-site sandwich radioimmunoassay of beta 2-microglobulin with monoclonal antibodies. J Immunol Methods. 1984 Dec 14;75 (1):43-51
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
  • Wibell L, Evrin PE, Berggard I. Serum 2 -microglobulin in renal disease. Nephron. 1973;10 (5):320-31
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