Total 242 AA. MW: 27.02 kDa (calculated). UniProtKB acc.no. Q9BXI9. N-terminal His-tag, 10extra AA (highlighted).
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95 %
12% SDS-PAGE separation of Human CTRP6:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. non-reduced and non-heated sample, 2.5μg / lane
3. reduced and heated sample, 2.5μg / lane
< 0.1 EU/μg
0.2 – 0.6 mg/ml in 0.05M acetate buffer, 5mM DTT pH=4.0 – filtered (0.4 μm), frozen
Defrost at ambient temperature.Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.