Type
Recombinant protein
Description
Total 242 AA. MW: 27.02 kDa (calculated). UniProtKB acc.no. Q9BXI9. N-terminal His-tag, 10 extra AA.
Amino Acid Sequence
MKHHHHHHASTFDRAVASGCQRCCDSEDPLDPAHVSSASSSGRPHALPEIRPYINITILKGDKGDPGPMGLPGYMGREGPQGEPGPQGSKGDKGEMGSPGAPCQKRFFAFSVGRKTALHSGEDFQTLLFERVFVNLDGCFDMATGQFAAPLRGIYFFSLNVHSWNYKETYVHIMHNQKEAVILYAQPSERSIMQSQSVMLDLAYGDRVWVRLFKRQRENAIYSNDFDTYITFSGHLIKAEDD
Source
E. coli
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
12% SDS-PAGE separation of Human CTRP6:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. non-reduced and non-heated sample, 2.5μg / lane
3. reduced and heated sample, 2.5μg / lane
Endotoxin
< 1.0 EU/µg
Formulation
0.3 – 0.8 mg/ml in 0.05M acetate buffer, 5mM DTT pH=4.0 – filtered (0.4 μm), frozen
Reconstitution
Defrost at ambient temperature.
Applications
Western blotting, ELISA
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store protein at -80°C. Protein remains stable until the expiry date when stored at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
Endotoxin level determination.
Note
This product is intended for research use only.