United States set
language
Menu Shopping cart $0 Search
Distributed product

EndoTrap® HD 5

  • Regulatory status:RUO
  • Type:EndoTrap®HD
Cat. No. Size Price


LET0023 5 ml settled resin $701,84
Product Details
Technical Data

Type

EndoTrap®HD

Description

EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate. The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.

EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, High-Definition Matrix is also available for small-scale purification as pre-filled columns. EndoTrap® HD is manufactured by LIONEX GmbH in Germany.

Available Products:

EndoTrap® HD 1/1
Item no. LET0009 1 x 1 ml column, 25 mL 5x Equilibration Buffer, 25 mL 5x Regeneration Buffer, 25 mL 5x Storage Buffer

EndoTrap® HD 5/1
Item no. LET0010 5 x 1 ml column, 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer, 125 mL 5x Storage Buffer

EndoTrap® HD 5
Item no. LET0023 5 mL settled resin

EndoTrap® HD 10
Item no. LET0011 10 mL settled resin

EndoTrap® HD 50
Item no. LET0012 50 ml settled resin

EndoTrap® HD 250
Item no. LET0013 250 ml settled resin

EndoTrap® HD Buffer Kit
Item no. LET0031 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer, 125 mL 5x Storage Buffer

EndoTrap® HD Leakage ELISA
Item no. LET0014 96 determinations (12 x 8 well strip & frame)

Empty Columns Large
Item no. LET0019 pack of 5 columns

Empty Columns Small
Item no. LET0020 pack of 5 columns

Disposable Polypropylene Funnel
Item no. LET0018 pack of 5 funnels

Summary

Features

  • Best-in-class endotoxin binding capacity of > 5,000,000 EU/ml
  • Removes more than 99.99% of LPS
  • Excellent sample recovery rate >95%
  • Non-toxic bacteriophage-derived ligand, no cytotoxic effects
  • Re-use more than 10 times
  • pH sample range: 4-10
  • suitable for high salt conditions
  • suitable for e.g. TRIS, HEPES, MOPS or similar buffered samples
  • PBS buffer possible when enriched freshly with 0.1 mM calcium (magnesium) directly before use
  • EndoTrap® Leakage ELISA for ligand leakage monitoring
  • Available as resin (buffers on request)
  • Developed by Hyglos GmbH and manufactured by LIONEX GmbH in Germany

Summary

EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.

The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.

EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.

Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided

Product References (26)

References

  • Chung E, Choi Y, Park J, Nah W, Park J, Jung Y, Lee J, Lee H, Park S, Hwang S, Kim S, Lee J, Min D, Jo J, Kang S, Jung M, Lee PH, Ruley HE, Jo D. Intracellular delivery of Parkin rescues neurons from accumulation of damaged mitochondria and pathological α-synuclein. Sci Adv. 2020 Apr 29;6(18):eaba1193. doi: 10.1126/sciadv.aba1193. eCollection 2020 May. PubMed PMID: 32494688. PubMed CentralPMCID: PMC7190327. See more on PubMed
  • Majewska J, Kaźmierczak Z, Lahutta K, Lecion D, Szymczak A, Miernikiewicz P, Drapała J, Harhala M, Marek-Bukowiec K, Jędruchniewicz N, Owczarek B, Górski A, Dąbrowska K. Induction of Phage-Specific Antibodies by Two Therapeutic Staphylococcal Bacteriophages Administered per os. Front Immunol. 2019 Nov 14;10:2607. doi: 10.3389/fimmu.2019.02607. eCollection 2019. PubMed PMID: 31803179. PubMed CentralPMCID: PMC6871536. See more on PubMed
  • Cafora M, Deflorian G, Forti F, Ferrari L, Binelli G, Briani F, Ghisotti D, Pistocchi A. Phage therapy against Pseudomonas aeruginosa infections in a cystic fibrosis zebrafish model. Sci Rep. 2019 Feb 6;9(1):1527. doi: 10.1038/s41598-018-37636-x. PubMed PMID: 30728389. PubMed CentralPMCID: PMC6365511. See more on PubMed
  • Bartling B, Rehfeld JS, Boßmann D, Vries I, Fohrer J, Lammers F, Scheper T, Beutel S. Determination of the Structural Integrity and Stability of Polysialic Acid during Alkaline and Thermal Treatment. Molecules. 2019 Dec 31;25(1):165. doi: 10.3390/molecules25010165. PubMed PMID: 31906121. PubMed CentralPMCID: PMC6982714. See more on PubMed
  • Rubalskii E, Ruemke S, Salmoukas C, Boyle EC, Warnecke G, Tudorache I, Shrestha M, Schmitto JD, Martens A, Rojas SV, Ziesing S, Bochkareva S, Kuehn C, Haverich A. Bacteriophage Therapy for Critical Infections Related to Cardiothoracic Surgery. Antibiotics (Basel). 2020 May 5;9(5):232. doi: 10.3390/antibiotics9050232. PubMed PMID: 32380707. PubMed CentralPMCID: PMC7277081. See more on PubMed
  • Kim NS, Torrez T, Langridge W. LPS enhances CTB-INSULIN induction of IDO1 and IL-10 synthesis in human dendritic cells. Cell Immunol. 2019 Apr;338:32-42. doi: 10.1016/j.cellimm.2019.03.003. Epub 2019 Mar 19. PubMed PMID: 30910218. PubMed CentralPMCID: PMC6481636. See more on PubMed
  • Sawamoto K, Tomatsu S. Development of Substrate Degradation Enzyme Therapy for Mucopolysaccharidosis IVA Murine Model. Int J Mol Sci. 2019 Aug 24;20(17):4139. doi: 10.3390/ijms20174139. PubMed PMID: 31450640. PubMed CentralPMCID: PMC6747109. See more on PubMed
  • Schmied BJ, Lutz MS, Riegg F, Zekri L, Heitmann JS, Bühring HJ, Jung G, Salih HR. Induction of NK Cell Reactivity against B-Cell Acute Lymphoblastic Leukemia by an Fc-Optimized FLT3 Antibody. Cancers (Basel). 2019 Dec 6;11(12):1966. doi: 10.3390/cancers11121966. PubMed PMID: 31817795. PubMed CentralPMCID: PMC6966676. See more on PubMed
  • Bell TJ, Brand OJ, Morgan DJ, Salek-Ardakani S, Jagger C, Fujimori T, Cholewa L, Tilakaratna V, Östling J, Thomas M, Day AJ, Snelgrove RJ, Hussell T. Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis. Matrix Biol. 2019 Jul;80:14-28. doi: 10.1016/j.matbio.2018.06.006. Epub 2018 Jun 20. PubMed PMID: 29933044. PubMed CentralPMCID: PMC6548309. See more on PubMed
  • Diéguez-Hurtado R, Kato K, Giaimo BD, Nieminen-Kelhä M, Arf H, Ferrante F, Bartkuhn M, Zimmermann T, Bixel MG, Eilken HM, Adams S, Borggrefe T, Vajkoczy P, Adams RH. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019 Jun 27;10(1):2817. doi: 10.1038/s41467-019-10643-w. PubMed PMID: 31249304. PubMed CentralPMCID: PMC6597568. See more on PubMed
  • Forti F, Roach DR, Cafora M, Pasini ME, Horner DS, Fiscarelli EV, Rossitto M, Cariani L, Briani F, Debarbieux L, Ghisotti D. Design of a Broad-Range Bacteriophage Cocktail That Reduces Pseudomonas aeruginosa Biofilms and Treats Acute Infections in Two Animal Models. Antimicrob Agents Chemother. 2018 May 25;62(6):e02573-17. doi: 10.1128/AAC.02573-17. Print 2018 Jun. PubMed PMID: 29555626. PubMed CentralPMCID: PMC5971607. See more on PubMed
  • Hietala V, Horsma-Heikkinen J, Carron A, Skurnik M, Kiljunen S. The Removal of Endo- and Enterotoxins From Bacteriophage Preparations. Front Microbiol. 2019 Jul 23;10:1674. doi: 10.3389/fmicb.2019.01674. eCollection 2019. PubMed PMID: 31396188. PubMed CentralPMCID: PMC6664067. See more on PubMed
  • Schmied BJ, Riegg F, Zekri L, Grosse-Hovest L, Bühring HJ, Jung G, Salih HR. An Fc-Optimized CD133 Antibody for Induction of Natural Killer Cell Reactivity against Colorectal Cancer. Cancers (Basel). 2019 Jun 7;11(6):789. doi: 10.3390/cancers11060789. PubMed PMID: 31181683. PubMed CentralPMCID: PMC6627285. See more on PubMed
  • Martin T, Möglich A, Felix I, Förtsch C, Rittlinger A, Palmer A, Denk S, Schneider J, Notbohm L, Vogel M, Geiger H, Paschke S, Huber-Lang M, Barth H. Rho-inhibiting C2IN-C3 fusion toxin inhibits chemotactic recruitment of human monocytes ex vivo and in mice in vivo. Arch Toxicol. 2018 Jan;92(1):323-336. doi: 10.1007/s00204-017-2058-y. Epub 2017 Sep 18. PubMed PMID: 28924833. PubMed CentralPMCID: PMC5773661. See more on PubMed
  • Hammond JW, Qiu WQ, Marker DF, Chamberlain JM, Greaves-Tunnell W, Bellizzi MJ, Lu SM, Gelbard HA. HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q. Glia. 2018 Dec;66(12):2563-2574. doi: 10.1002/glia.23511. Epub 2018 Oct 16. PubMed PMID: 30325063. PubMed CentralPMCID: PMC6309507. See more on PubMed
  • Trend S, Chang BJ, O'Dea M, Stick SM, Kicic A. Use of a Primary Epithelial Cell Screening Tool to Investigate Phage Therapy in Cystic Fibrosis. Front Pharmacol. 2018 Nov 28;9:1330. doi: 10.3389/fphar.2018.01330. eCollection 2018. PubMed PMID: 30546305. PubMed CentralPMCID: PMC6280614. See more on PubMed
  • Müller HD, Caballé-Serrano J, Lussi A, Gruber R. Inhibitory effect of saliva on osteoclastogenesis in vitro requires toll-like receptor 4 signaling. Clin Oral Investig. 2017 Nov;21(8):2445-2452. doi: 10.1007/s00784-016-2041-7. Epub 2017 Jan 18. PubMed PMID: 28101679. PubMed CentralPMCID: PMC5632348. See more on PubMed
  • Müller HD, Cvikl BB, Lussi AA, Gruber RR. Salivary pellets induce a pro-inflammatory response involving the TLR4-NF-kB pathway in gingival fibroblasts. BMC Oral Health. 2016 Jul 8;17(1):15. doi: 10.1186/s12903-016-0229-5. PubMed PMID: 27430277. PubMed CentralPMCID: PMC4948095. See more on PubMed
  • Almeida APMM, Machado LFM, Doro D, Nascimento FC, Damasceno L, Gazzinelli RT, Fernandes AP, Junqueira C. New Vaccine Formulations Containing a Modified Version of the Amastigote 2 Antigen and the Non-Virulent Trypanosoma cruzi CL-14 Strain Are Highly Antigenic and Protective against Leishmania infantum Challenge. Front Immunol. 2018 Mar 15;9:465. doi: 10.3389/fimmu.2018.00465. eCollection 2018. PubMed PMID: 29599776. PubMed CentralPMCID: PMC5863692. See more on PubMed
  • Orellana JM, Kampe K, Schulze F, Sieber J, Jehle AW. Fetuin-A aggravates lipotoxicity in podocytes via interleukin-1 signaling. Physiol Rep. 2017 May;5(10):e13287. doi: 10.14814/phy2.13287. PubMed PMID: 28554965. PubMed CentralPMCID: PMC5449566. See more on PubMed
  • Rojas JM, Arán-Sekul T, Cortés E, Jaldín R, Ordenes K, Orrego PR, González J, Araya JE, Catalán A. Phospholipase D from Loxosceles laeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration. Toxins (Basel). 2017 Apr 5;9(4):125. doi: 10.3390/toxins9040125. PubMed PMID: 28379166. PubMed CentralPMCID: PMC5408199. See more on PubMed
  • Kłopot A, Zakrzewska A, Lecion D, Majewska JM, Harhala MA, Lahutta K, Kaźmierczak Z, Łaczmański Ł, Kłak M, Dąbrowska K. Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles. Front Microbiol. 2017 Nov 6;8:2170. doi: 10.3389/fmicb.2017.02170. eCollection 2017. PubMed PMID: 29163448. PubMed CentralPMCID: PMC5672142. See more on PubMed
  • Kwon MS, Woo SK, Kurland DB, Yoon SH, Palmer AF, Banerjee U, Iqbal S, Ivanova S, Gerzanich V, Simard JM. Methemoglobin is an endogenous toll-like receptor 4 ligand-relevance to subarachnoid hemorrhage. Int J Mol Sci. 2015 Mar 5;16(3):5028-46. doi: 10.3390/ijms16035028. PubMed PMID: 25751721. PubMed CentralPMCID: PMC4394463. See more on PubMed
  • Campbell IK, Miescher S, Branch DR, Mott PJ, Lazarus AH, Han D, Maraskovsky E, Zuercher AW, Neschadim A, Leontyev D, McKenzie BS, Käsermann F. Therapeutic effect of IVIG on inflammatory arthritis in mice is dependent on the Fc portion and independent of sialylation or basophils. J Immunol. 2014 Jun 1;192(11):5031-8. doi: 10.4049/jimmunol.1301611. Epub 2014 Apr 23. PubMed PMID: 24760152. PubMed CentralPMCID: PMC4025610. See more on PubMed
  • Cattepoel S, Schaub A, Ender M, Gaida A, Kropf A, Guggisberg U, Nolte MW, Fabri L, Adlard PA, Finkelstein DI, Bolli R, Miescher SM. Intravenous immunglobulin binds beta amyloid and modifies its aggregation, neurotoxicity and microglial phagocytosis in vitro. PLoS One. 2013 May 16;8(5):e63162. doi: 10.1371/journal.pone.0063162. Print 2013. PubMed PMID: 23696796. PubMed CentralPMCID: PMC3656042. See more on PubMed
  • Käsermann F, Boerema DJ, Rüegsegger M, Hofmann A, Wymann S, Zuercher AW, Miescher S. Analysis and functional consequences of increased Fab-sialylation of intravenous immunoglobulin (IVIG) after lectin fractionation. PLoS One. 2012;7(6):e37243. doi: 10.1371/journal.pone.0037243. Epub 2012 Jun 4. PubMed PMID: 22675478. PubMed CentralPMCID: PMC3366990. See more on PubMed
Docs