Total 207 AA. MW: 23.1 kDa (calculated). N-Terminal His-tag (10 extra AA). UniprotKB acc.no. P98173. Protein identity confirmed by LC-MS/MS. Identification of recombinant FAM3A by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95%
12% SDS-PAGE separation of Human FAM3A:
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 2.5μg / lane
3. non-reduced and non-boiled sample, 2.5μg / lane
< 0.1 EU/μg
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 50 mM Tris buffer, pH 7.5, containing 0.1M arginine.
Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.