Total 207 AA. MW: 23.1 kDa (calculated). N-Terminal His-tag (10 extra AA). UniprotKB acc.no. P98173. Protein identity confirmed by MS. Identification of recombinant FAM3A by MS.
Amino Acid Sequence
˃ 90 % by SDS-PAGE
12% SDS-PAGE separation of Human FAM3A:
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 2.5μg / lane
3. non-reduced and non-boiled sample, 2.5μg / lane
< 1.0 EU/µg
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 50 mM Tris buffer, pH 7.5, containing 0.1M arginine.
Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
Endotoxin level determination.
This product is intended for research use only.