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FAM3A Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Cytokine-lie protein 2-19
  • Species:Human
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RD172301100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 207 AA. MW: 23.1 kDa (calculated). N-Terminal His-tag (10 extra AA). UniprotKB acc.no. P98173. Protein identity confirmed by LC-MS/MS. Identification of recombinant FAM3A by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHASFPRIQQLFTSPESSVTAAPRARKYKCGLPQPCPEEHLAFRVVSGAANVIGPKICLEDKMLMSSVKDNVGRGLNIALVNGVSGELIEARAFDMWAGDVNDLLKFIRPLHEGTLVFVASYDDPATKMNEETRKLFSELGSRNAKELAFRDSWVFVGAKGVQNKSPFEQHVKNSKHSNKYEGWPEALEMEGCIPRRSTAS

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human FAM3A:
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and boiled sample, 2.5μg / lane
3. non-reduced and non-boiled sample, 2.5μg / lane

Endotoxin

< 1.0 EU/µg

Formulation

Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 100mM TRIS pH 7.5 containing 0.1M arginine.

Reconstitution

Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.

Quality Control Test

Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.

Note

This product is intended for research use only.

Summary

Research topic

Others

Summary

Function of the FAM3A protein is unclear up to now. It belongs to the FAM3 family along with FAM3B (Pander) protein which induces apoptosis of pancreatic alpha and beta cells. FAM3A protein is expressed in almost all tissues.

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