Type
Native protein
Description
Native protein isolated from human pooled plasma, 349 AA, MW: 37.3 kDa (calculated without glycosylation). Protein identity confirmed by MS.
Source
Human plasma
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
14% SDS-PAGE separation of Human Fetuin
1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
Endotoxin
< 1.0 EU/ug
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in 20 mM Tris buffer, 200 mM NaCl, pH 8.0
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Filter your culture media/working solutions containing this product before using in cell culture.
Applications
Western blotting, ELISA
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Endotoxin level determination.
Note
All blood samples used for protein preparation were tested and found negative for HBsAg, anti-HCV, anti-HBc and anti-HIV 1 and 2. Since no test can absolutely assure the absence of all infectious agents, this product should be handled as a potential biohazard. This product is intended for research use only.