Human Lipopolysaccharide Binding Protein (LBP) ELISA

Features

It is intended for research use only

The total assay time is less than 3 hours

The kit measures specifically Lipopolysaccharide Binding Protein

Assay format is 96 wells

Quality Controls are human serum based

Standard is a recombinant protein

Components of the kit are provided ready to use, concentrated or dried

Research topic

Immune Response, Infection and Inflammation, Sepsis

Summary

Lipopolysaccharide binding protein (LBP, LPS-binding protein) is a serum glycoprotein belonging to the family of lipid-binding proteins. LBP is synthesized by hepatocytes and intestinal epithelial cells. Serum concentrations of LBP range between 5 and 10 ug/ml during homeostasis, increasing up to 200 ug/ml during the acute-phase response in the course of infection. LBP expression and function are strongly associated with recognition and control of bacterial infection. LBP is an acute-phase reactant. As a class I acute-phase protein, LBP is induced by pro-inflammatory cytokines such as interleukins 1 and 6, tumor necrosis factor-alpha (TNF-α), and glucocorticoid hormones in liver and in non-hepatic tissues such as the gut and the lung. LPS is released from CD14 in the lipid bilayer and binds to a complex of receptors including Toll-like receptor 4 (TLR-4) to initiate intracellular signaling cascades and transcription of genes mediated through NF-κB. If dysregulated, these host reactions can have inadvertent outcomes such as severe sepsis, septic shock, or systemic inflammatory response syndrome (SIRS). LBP binds LPS in various pathologic states including obesity and insulin resistance.