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Human Phosphotyrosine Tie-2 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Phosphotyrosine Tie-2
  • Species:Human
Cat. No. Size Price


New REH012R 96 wells (1 kit) $518,5
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell lysate

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Summary

Features

  • RUO
  • positive control

Summary

The BioVendor Phosphotyrosine TIE-2 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated TIE-2 in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis. This sandwich-based ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human phospho-TIE-2. An anti-TIE-2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated TIE-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-TIE-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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