Human Proguanylin ELISA

Cat. No.	Size	Price
New RBL029R	96 wells (1 kit)

Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma

Sample Requirements

10 µl/well

Shipping

Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

10 - 0,156 ng/ml

Limit of Detection

5 pg/ml

Intra-assay (Within-Run)

CV =8,3%

Inter-assay (Run-to-Run)

CV =11,3%

Spiking Recovery

98%

Dilution Linearity

90%

Summary

Features

It is intended for research use only
The total assay time is less than 3 hours
The kit measures specifically human Proguanylin
Assay format is 96 wells
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried

Research topic

Autoimmunity, Blood pressure regulation and NO metabolism, Others, Renal disease

Summary

Proguanylin (guanylate cyclase activator 2A, guanylate cyclase-activating protein 1), is a precursor to the hormone guanylin, and plays a significant role in various physiological processes in the kidney and intestine through activation of guanylate cyclase C receptor. In Crohn's disease, serum proguanylin levels are notably reduced compared to healthy individuals, and levels increase following anti-inflammatory treatment, suggesting its potential as a biomarker for disease activity and therapeutic response. In the context of hypertension, elevated proguanylin levels have been observed in individuals with high blood pressure, indicating a possible role in sodium metabolism and blood pressure regulation.

Summary References (7)

References to Proguanylin

Fan X, Wang Y, London R M, Eber S L, Kruse W J, Freeman R H, and Forte L R: Signaling Pathways for Guanylin and Uroguanylin in the digestive, Renal, Central Nervous, Reproductive, and Lymphoid Systems. Endocrinology 138, 4636–4648 (1997)
Garcia k Ch, Sauvage F J, Strubles M, Henzel W, Reilly D, and Goeddel D V: Processing and Charecterization of Human Proguanylin Expressed in Escherichia coli. The Journal of Biological Chemistry 268, 22397–22401 (1993)
Hamra F K, Fan X, Krause W J, et al: Prouroguanylin and Proguanylin: Purification from Colon, Structure, and Modulation of Bioactivyity by Proteases. Endocrinology 137, 257–265 (1996)
Rudolph J A, Hawkins J A, and Cohen M B: Proguanylin secretion and role of negative – feedback inhibition in villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol 238 G695-G702 (2002)
Lauber T, Neudecker P, Rosch P, and Marx U C: Solution Structure of Human Proguanylin. The Journal of Biological Chemistry 278, 24118–24124 (2003)
Lauber T, Nourse A, Schulz A, and Marx U C: Native and Recombinant Proguanylin Feature Identical Biophysical Properties and Are Monomeric in Solution. Biochemistry 41, 14602–14612 (2002)
Sauvage J F, Keshav S, Kuang W-J, Gillett N, Henzel W, and Goeddel D V: Precursor structure, expression, and tissue distribution of human guanylin. Biochemistry 89, 9089–9093 (1992)

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