Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
10 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.1875 - 6 ng/ml
Limit of Detection
20 pg/ml
Intra-assay (Within-Run)
CV =6.9%
Inter-assay (Run-to-Run)
CV =12.9%
Spiking Recovery
113%
Dilution Linearity
96%
Features
- It is intended for research use only
- The total assay time is less than 2.5 hours
- The kit measures specifically human sST2
- Assay format is 96 wells
- Standard is a recombinant protein
- Components of the kit are provided ready to use, concentrated or dried
Research topic
Cardiovascular disease, Coronary artery disease
Summary
sST2 (Soluble suppression of tumorigenicity 2) is a member of the interleukin-1 receptor family. Like other members of the IL-1 family, the binding of IL-33 to its transmembrane receptor ST2L on the surface of inflammatory cells activates intracellular signaling pathways and mediates pro-inflammatory effects. The soluble ST2 isoform is released by activated Th2 cells and stressed cardiac myocytes and is elevated in the serum of patients with allergic asthma. Soluble ST2 acts as a decoy receptor, blocking IL‑33’s ability to signal through the transmembrane ST2 receptor. sST2 is a strong predictor of disease severity and mortality in cardiovascular disease and serves as a reliable predictor of mortality in patients with stable coronary artery disease and chronic heart failure.
