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Manufactured by BioVendor

Rat Insulin Elisa High Sensitive

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Rat
Cat. No. Size Price


RAI006R 96 wells (1 kit) $552
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Description

This Rat Insulin High Senstivity ELISA kit is for the quantitative determination of insulin in rat serum, plasma and fluid.

Applications

Serum, Plasma, Body fluids

Sample Requirements

10 µl

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Curve

Calibration Range

0.2 – 7 ng/ml

Limit of Detection

0.2 ng/ml

Intra-assay (Within-Run)

CV < 6.1%

Inter-assay (Run-to-Run)

CV <2.9%

Spiking Recovery

91%

Summary

Features

  • RUO
  • calibration range 0.2-7 ng/ml
  • limit of detection 0.2 ng/ml
  • intra-assay CV < 6.1%
  • inter-assay CV < 2.9 %

Research topic

Diabetology - Insulin, C-Peptide, Proinsulin, Energy metabolism and body weight regulation, Animal studies

Summary

This Rat Insulin High Senstivity ELISA kit is for the quantitative determination of insulin in rat serum, plasma and fluid.

Summary References (8)

References to Insulin

  • Flier JS, Kahn CR, Roth J. Receptors, antireceptor antibodies and mechanisms of insulin resistance. N Engl J Med. 1979 Feb 22;300 (8):413-9
  • Frier BM, Ashby JP, Nairn IM, Baird JD. Plasma insulin, C-peptide and glucagon concentrations in patients with insulin-independent diabetes treated with chlorpropamide. Diabete Metab. 1981 Mar;7 (1):45-9
  • Judzewitsch RG, Pfeifer MA, Best JD, Beard JC, Halter JB, Porte D Jr. Chronic chlorpropamide therapy of noninsulin-dependent diabetes augments basal and stimulated insulin secretion by increasing islet sensitivity to glucose. J Clin Endocrinol Metab. 1982 Aug;55 (2):321-8
  • Kosaka K, Hagura R, Kuzuya T. Insulin responses in equivocal and definite diabetes, with special reference to subjects who had mild glucose intolerance but later developed definite diabetes. Diabetes. 1977 Oct;26 (10):944-52
  • Starr JI, Mako ME, Juhn D, Rubenstein AH. Measurement of serum proinsulin-like material: cross-reactivity of porcine and human proinsulin in the insulin radioimmunoassay. J Lab Clin Med. 1978 Apr;91 (4):683-92
  • Temple R, Clark PM, Hales CN. Measurement of insulin secretion in type 2 diabetes: problems and pitfalls. Diabet Med. 1992 Jul;9 (6):503-12
  • Temple RC, Carrington CA, Luzio SD, Owens DR, Schneider AE, Sobey WJ, Hales CN. Insulin deficiency in non-insulin-dependent diabetes. Lancet. 1989 Feb 11;1 (8633):293-5
  • Temple RC, Clark PM, Nagi DK, Schneider AE, Yudkin JS, Hales CN. Radioimmunoassay may overestimate insulin in non-insulin-dependent diabetics. Clin Endocrinol (Oxf). 1990 Jun;32 (6):689-93
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