Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Omentin-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Intelectin-1, ITLN-1, Intestinal lactoferrin receptor, Galactofuranose-binding lectin, Endothelial lectin HL-1, Omentin, INTL, ITLN, LFR, UNQ640/PRO1270
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD191100200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

6 ul/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2–64 ng/ml

Limit of Detection

0.5 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 3.7%

Inter-assay (Run-to-Run)

n = 8; CV = 4.6%

Spiking Recovery

94,80%

Dilutation Linearity

104,10%

Crossreactivity

  • bovine Non-detectable
  • cat Not tested
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Not tested
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • monkey Strong reactivity (recom. dilution 1:80)
Summary

Features

  • It is intended for research use only.
  • The total assay time is less than 3.5 hours.
  • The kit measures omentin-1 in serum and plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Quality Controls are human serum based. No animal sera are used.
  • Standard is recombinant protein based.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction

Summary

Omentin (intelectin-1, intestinal lactoferin receptor, endothelian lectin HL-1, galactofurano­sebinding lectin) is newly identified secretory protein that is highly and selectively expressed in visceral adipose tissue relative to subcutaneous adipose tissue (adipokine). The mature omentin is secretory glycoprotein consisting of 295 amino acids and 1-linked oligosacharides, and its basic structural unit is a 120-kDa homotrimer in which 40-kDa polipeptides are bridged by disulfide bonds. Omentin has been identified in other tissue at lower expression levels such are Paneth cells, endothelial cells, and visceral adipose stromal-vascular cells. A homolog of omentin has been identified that shares 83% amino acid identity with omentin and was referred to as omentin 2. The two omentin genes, omentin 1 and omentin 2, are localized adjacent to each other in chromosomal region, which has been previously linked to type 2 diabetes in several populations. To determine the impact of obesity-dependent insulin resistance on the regulation of two omentin isoforms, gene expression and plasma levels were measured in lean, overweight, and obese subjects. Omentin-1 was shown to be the major circulating isoform in human plasma. Lean subjects had significantly higher omentin-1 plasma levels than obese and overweight subjects. In addition, higher plasma omentin-1 levels were detected in women compared with men. Plasma omentin-1 levels were inversely correlated with BMI, waist circumference, leptin levels, and insulin resistance as measured by homeostasis model assessment and positively correlated with adiponectin and HDL levels. In summary, decreased omentin-1 levels are associated with increasing obesity and insulin resistance. An independent experiment reported that the addition of recombinant omentin-1 in vitro did not affect basal glucose uptake but did enhance insulin-stimulated glucose uptake in both subcutaneous and omental human adipocytes. Omentin-1 increased Akt phosphorylation in the absence and presence of insulin and may regulate insulin action. A recent study of women with the polycystic ovary syndrome (PCOS) found significantly reduced omentin-1 mRNA expression and protein levels in adipose tissue in overweight PCOS women. In addition, significantly lower plasma omentin-1 levels were detected in these women.

References to Product

References

  • Al-Kuraishy HM, Hamada MT, Al-Samerraie AY. Effects of metformin on omentin levels in a newly diagnosed type II diabetes mellitus: Randomized, placebo controlled study. Mustansiriya Medical Journa. April 2016;15 (1):49-55
  • Aleksandrova K, di Giuseppe R, Isermann B, Biemann R, Schulze M, Wittenbecher C, Fritsche A, Lehmann R, Menzel J, Weikert C, Pischon T, Boeing H. Circulating Omentin as a Novel Biomarker for Colorectal Cancer Risk: Data from the EPIC-Potsdam Cohort Study. Cancer Res. 2016 Jul 1;76 (13):3862-71
  • Alissa EM, Maisa'a MA, Alama NA, Ferns GA. Role of omentin-1 and C-reactive protein in obese subjects with subclinical inflammation. Journal of Clinical & Translat. March 2016;3:7-11
  • Berstein LM, ORCID: 0000-0002-5112-3372, Iyevleva AG, Mukhina MS, Vasilyev DA, Poroshina TE. Features of omental adipose tissue in endometrial cancer patients with 'standard' or 'metabolically healthy' obesity: associations with tumor process characteristics. Springerplus. 2016;5 (1):1900
  • Bienertová-Vašků J, Novák J, Zlámal F, Forejt M, Havlenová S, Jackowská A, Tomandl J, Tomandlová M, Šplíchal Z, Vašků A. The prediction role of indexes of circulating adipokines for common anthropometric and nutritional characteristics of obesity in the obese Central European population. Eating Behaviors. 2, April 2014;15:244-251
  • Canan F, Yildirim O, Tosun M, Kayka N, Tuman TC, Alcelik A. Serum levels of omentin are not altered in drug-naive patients with major depression: a pilot study. Psychiatr Danub. 2014 Mar;26 (1):34-8
  • El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease. Diabet Med. 2011 Oct;28 (10):1194-200
  • Flehmig G, Scholz M, Kloting N, Fasshauer M, Tonjes A, Stumvoll M, Youn BS, Bluher M. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation. PLoS One. 2014;9 (6):e99785
  • Fryczkowski M, Bułdak RJ, Hejmo T, Kukla M, Żwirska-Korczala K. Circulating Levels of Omentin, Leptin, VEGF, and HGF and Their Clinical Relevance with PSA Marker in Prostate Cancer. Dis Markers. 2018;2018:3852401. Published 2018 Aug 16. doi:10.1155/2018/3852401
  • Hernández EÁ, Kahl S, Seelig A, Begovatz P, Irmler M, Kupriyanova Y, Nowotny B, Nowotny P, Herder C, Barosa C, Carvalho F, Rozman J, Neschen S, Jones JG, Beckers J, de Angelis MH, Roden M. Acute dietary fat intake initiates alterations in energy metabolism and insulin resistance. J Clin Invest. 2017 Feb 1;127(2):695-708. doi: 10.1172/JCI89444
  • Huang CJ, Beasley KN, Acevedo EO, Franco RL, Jones TL, Mari DC, Shibata Y. Chitin enhances obese inflammation ex vivo. Hum Immunol. 2013 Sep 17;
  • Choi JH, Rhee EJ, Kim KH, Woo HY, Lee WY, Sung KC. Plasma omentin-1 levels are reduced in non-obese women with normal glucose tolerance and polycystic ovary syndrome. Eur J Endocrinol. 2011 Nov;165 (5):789-96
  • Kadoglou NP, Lambadiari V, Gastounioti A, Gkekas C, Giannakopoulos TG, Koulia K, Maratou E, Alepaki M, Kakisis J, Karakitsos P, Nikita KS, Dimitriadis G, Liapis CD. The relationship of novel adipokines, RBP4 and omentin-1, with carotid atherosclerosis severity and vulnerability. Atherosclerosis. 2014 Aug;235 (2):606-12
  • Kafalidis G, Boutsikou T, Briana DD, Boutsikou M, Marmarinos A, Baka S, Hassiakos D, Gourgiotis D, Malamitsi-Puchner A. Adipokines vaspin and omentin-1 are up-regulated in large for gestational age infants at term. Cytokine. 2013 Apr;62 (1):70-4
  • Kataoka Y, Shibata R, Ohashi K, Kambara T, Enomoto T, Uemura Y, Ogura Y, Yuasa D, Matsuo K, Nagata T, Oba T, Yasukawa H, Numaguchi Y, Sone T, Murohara T, Ouchi N. Omentin prevents myocardial ischemic injury through AMPK- and Akt-dependent mechanisms. J Am Coll Cardiol. 2014 Apr 11;
  • Lesna J, Ticha A, Hyspler R, Blaha V, Musil F, Sobotka L, Smahelova A. Impact of weight reduction on plasma omentin-1 levels in obese patients with diabetes mellitus type 1. Nutrition. March 2013;29 (3):588
  • Lesna J, Ticha A, Hyspler R, Blaha V, Musil F, Sobotka L, Smahelova A. PP205-MON IMPACT OF LONG-TERM DIET CHANGES ON PLASMA OMENTIN-1 LEVELS IN OBESE PATIENTS WITH DIABETES MELLITUS TYPE 1. Clinical Nutrition Supplements. September 2012;7 (1):219
  • Lesná J, Tichá A, Hyšpler R, et al. Omentin-1 plasma levels and cholesterol metabolism in obese patients with diabetes mellitus type 1: impact of weight reduction. Nutr Diabetes. 2015;5(11):e183. Published 2015 Nov 2. doi:10.1038/nutd.2015.33
  • Luque-Ramirez M, Martinez-Garcia MA, Montes-Nieto R, Fernandez-Duran E, Insenser M, Alpanes M, Escobar-Morreale HF. Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge. Hum Reprod. 2013 Jul;28 (7):1908-18
  • Maruyama S, Shibata R, Kikuchi R, Izumiya Y, Rokutanda T, Araki S, Kataoka Y, Ohashi K, Daida H, Kihara S, Ogawa H, Murohara T, Ouchi N. Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via endothelial nitric oxide synthase-dependent mechanism. J Biol Chem. 2012 Jan 2;287 (1):408-17
  • Menzel J, Di Giuseppe R, Biemann R, Aleksandrova K, Kuxhaus O, Wittenbecher C, Fritsche A, Schulze MB, Isermann B, Boeing H, Weikert C. Association between omentin-1, adiponectin and bone health under consideration of osteoprotegerin as possible mediator. J Endocrinol Invest. 2016 Sep 10;
  • Menzel J, di Giuseppe R, Biemann R, Wittenbecher C, Aleksandrova K, Pischon T, Fritsche A, Schulze MB, Boeing H, Isermann B, Weikert C. Omentin-1 and risk of myocardial infarction and stroke: Results from the EPIC-Potsdam cohort study. Atherosclerosis. 2016 Aug;251:415-21
  • Mierzyński R, Dłuski D, Nowakowski Ł, Poniedziałek-Czajkowska E, Leszczyńska-Gorzelak B. Adiponectin and Omentin Levels as Predictive Biomarkers of Preterm Birth in Patients with Gestational Diabetes Mellitus. Biomed Res Int. 2018;2018:7154216. Published 2018 Sep 18. doi:10.1155/2018/7154216
  • Ochman M, Maruszewski M, Wojarski J, et al. Serum Levels of Visfatin, Omentin and Irisin in Patients with End-Stage Lung Disease Before and After Lung Transplantation. Ann Transplant. 2017;23:765–772. Published 2017 Dec 26. doi:10.12659/AOT.904994
  • Orlik B, Madej P, Owczarek A, Skalba P, Chudek J, Olszanecka-Glinianowicz M. Plasma omentin and adiponectin levels as markers of adipose tissue dysfunction in normal weight and obese women with polycystic ovary syndrome. Clin Endocrinol (Oxf). 2014 Oct;81 (4):529-35
  • Panagiotou G, Mu L, Na B, Mukamal KJ, Mantzoros CS. Circulating irisin, omentin-1, and lipoprotein subparticles in adults at higher cardiovascular risk. Metabolism. 2014 Oct;63 (10):1265-71
  • Peraire J, López-Dupla M, Alba V, Beltrán-Debón R, Martinez E, Domingo P, Asensi V, Leal M, Viladés C, Inza MI, Escoté X, Arnedo M, Mateo G, Valle-Garay E, Ferrando-Martinez S, Veloso S,Vendrell J, Gatell JM, Vidal F. HIV/antiretroviral therapy–related lipodystrophy syndrome (HALS) is associated with higher RBP4 and lower omentin in plasma, Clinical Microbiology and Infection. CMI. July 2015;21 (7):711.e1-711.e8
  • Rozmilowska I, Czyzewski D, Mazur B, Adamczyk-Sowa M. What is the role of adipokines in myasthenia gravis?. Ther Clin Risk Manag. 2018;14:1515-1525. Published 2018 Aug 28. doi:10.2147/TCRM.S163966
  • Shibata R, Ouchi N, Kikuchi R, Takahashi R, Takeshita K, Kataoka Y, Ohashi K, Ikeda N, Kihara S, Murohara T. Circulating omentin is associated with coronary artery disease in men. Atherosclerosis. 2011 Dec;219 (2):811-4
  • Shibata R, Ouchi N, Takahashi R, Terakura Y, Ohashi K, Ikeda N, Higuchi A, Terasaki H, Kihara S, Murohara T. Omentin as a novel biomarker of metabolic risk factors. Diabetol Metab Syndr. 2012;4 (1):37
  • Shibata R, Takahashi R, Kataoka Y, Ohashi K, Ikeda N, Kihara S, Murohara T, Ouchi N. Association of a fat-derived plasma protein omentin with carotid artery intima-media thickness in apparently healthy men. Hypertens Res. 2011 Dec;34 (12):1309-12
  • Siejka A, Jankiewicz-Wika J, Kolomecki K, Cywinski J, Piestrzeniewicz K, Swietoslawski J, Stepien H, Komorowski J. Long-term impact of vertical banded gastroplasty (VBG) on plasma concentration of leptin, soluble leptin receptor, ghrelin, omentin-1, obestatin, and retinol binding protein 4 (RBP4) in patients with severe obesity. Cytokine. 2013 Nov;64 (2):490-3
  • Soliman PT, Zhang Q, Broaddus RR, Westin SN, Iglesias D, Munsell MF, Schmandt R, Yates M, Ramondetta L, Lu KH. Prospective evaluation of the molecular effects of metformin on the endometrium in women with newly diagnosed endometrial cancer: A window of opportunity study. Gynecol Oncol. 2016 Oct 13;
  • Urbanova M, Dostalova I, Trachta P, Drapalova J, Kavalkova P, Haluzikova D, Matoulek M, Lacinova Z, Mraz M, Kasalicky M, Haluzik M. Serum concentrations and subcutaneous adipose tissue mRNA expression of omentin in morbid obesity and type 2 diabetes mellitus: the effect of very-low-calorie diet, physical activity and laparoscopic sleeve gastrectomy. Physiol Res. 2014;63 (2):207-18
  • Watanabe K, Watanabe R, Konii H, Shirai R, Sato K, Matsuyama TA, Ishibashi-Ueda H, Koba S, Kobayashi Y, Hirano T, Watanabe T. Counteractive effects of omentin-1 against atherogenesisdagger. Cardiovasc Res. 2016 May 1;110 (1):118-28
  • Wijetunge S, Ratnayake RMCJ, Kotakadeniya HMSRB, Rosairo S, Albracht-Schulte K, Ramalingam L, Moustaid-Moussa N, Kalupahana NS. Association between serum and adipose tissue resistin with dysglycemia in South Asian women. Nutr Diabetes. 2019 Feb 18;9(1):5. doi: 10.1038/s41387-019-0071-3.
  • Wittenbecher C, Menzel J, ORCID: http://orcid.org/0000-0001-7792-877X, Carstensen-Kirberg M, Biemann R, di Giuseppe R, Fritsche A, Isermann B, Herder C, Aleksandrova K, Boeing H, Weikert C, Schulze MB. Omentin-1, Adiponectin, and the Risk of Developing Type 2 Diabetes. Diabetes Care. 2016 Jun;39 (6):e79-80
  • Wojcik K, Jablonowska E, Omulecka A, Piekarska A. Insulin resistance, adipokine profile and hepatic expression of SOCS-3 gene in chronic hepatitis C. World J Gastroenterol. 2014 Aug 14;20 (30):10449-56
  • Wyskida K, Franik G, Wikarek T, et al. The levels of adipokines in relation to hormonal changes during the menstrual cycle in young, normal-weight women. Endocr Connect. 2017;6(8):892-900.
References to Summary

References to Omentin-1

  • de Souza Batista CM, Yang RZ, Lee MJ, Glynn NM, Yu DZ, Pray J, Ndubuizu K, Patil S, Schwartz A, Kligman M, Fried SK, Gong DW, Shuldiner AR, Pollin TI, McLenithan JC. Omentin plasma levels and gene expression are decreased in obesity. Diabetes. 2007 Jun;56 (6):1655-61
  • Moreno-Navarrete JM, Catalan V, Ortega F, Gomez-Ambrosi J, Ricart W, Fruhbeck G, Fernandez-Real JM. Circulating omentin concentration increases after weight loss. Nutr Metab (Lond). 2010;7:27
  • Pan HY, Guo L, Li Q. Changes of serum omentin-1 levels in normal subjects and in patients with impaired glucose regulation and with newly diagnosed and untreated type 2 diabetes. Diabetes Res Clin Pract. 2010 Apr;88 (1):29-33
  • Schaffler A, Neumeier M, Herfarth H, Furst A, Scholmerich J, Buchler C. Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue. Biochim Biophys Acta. 2005 Dec 30;1732 (1-3):96-102
  • Tan BK, Adya R, Farhatullah S, Lewandowski KC, O'Hare P, Lehnert H, Randeva HS. Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes. 2008 Apr;57 (4):801-8
  • Tan BK, Adya R, Farhatullah S, Lewandowski KC, O'Hare P, Lehnert H, Randeva HS. Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes. 2008 Apr;57 (4):801-8
  • Wurm S, Neumeier M, Weigert J, Schaffler A, Buechler C. Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults. Cardiovasc Diabetol. 2007;6:7
  • Yamawaki H, Tsubaki N, Mukohda M, Okada M, Hara Y. Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels. Biochem Biophys Res Commun. 2010 Mar 19;393 (4):668-72
  • Yang RZ, Lee MJ, Hu H, Pray J, Wu HB, Hansen BC, Shuldiner AR, Fried SK, McLenithan JC, Gong DW. Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action. Am J Physiol Endocrinol Metab. 2006 Jun;290 (6):E1253-61
  • Yilmaz Y, Yonal O, Kurt R, Alahdab YO, Eren F, Ozdogan O, Celikel CA, Imeryuz N, Kalayci C, Avsar E. Serum levels of omentin, chemerin and adipsin in patients with biopsy-proven nonalcoholic fatty liver disease. Scand J Gastroenterol. 2011 Jan;46 (1):91-7
Related Products Documents