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Manufactured by BioVendor

Pentraxin 3 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:PTX3, TSG-14, Pentraxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG14, TSG 14
  • Species:Human
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Cat. No. Size Price


New RD191477200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Stool, Serum, Saliva, Plasma

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

78 – 5000 pg/ml

Limit of Detection

22 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 3.4%

Inter-assay (Run-to-Run)

n = 6; CV = 7.2%

Spiking Recovery

92,70%

Dilutation Linearity

97,30%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures PTX3 in serum, plasma (EDTA, citrate, heparin), saliva and stool extract
  • For protocol for preparation of stool extracts and other details, please contact us at info@biovendor.com
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cardiovascular disease, Immune Response, Infection and Inflammation

Summary

Pentraxin 3 (PTX3, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein) is an evolutionarily conserved, multimeric acute phase inflammatory glycoprotein in the same family as the well-established cardiovascular biomarker C-reactive protein (CRP). It is the prototypical long pentraxin, exhibiting a C-terminal pentraxin domain characteristic of the family, and a unique N-terminal domain [1,13]. PTX3 is mainly produced at extrahepatic sites by several cell types, including cells of the myelomonocyte lineage (monocytes, macrophages, dendritic cells), endothelial cells, smooth muscle cells, fibroblasts, and adipocytes.
PTX3 is also produced during neutrophil differentiation and stored in specific granules of mature neutrophils, ready to be released upon microbial recognition. PTX3 behaves as an acute phase response protein, as the blood levels of PTX3, low in normal conditions (<2 ng/ml in humans), increase rapidly (peaking at 6–8 hours after induction) and dramatically (200–800 ng/ml) during endotoxic shock, sepsis, and other inflammatory and infectious conditions, correlating with the severity of the disease. PTX3 has multiple complex nonredundant functions, ranging from assembly of a hyaluronic acid–rich extracellular matrix and female fertility to protection against pathogens (i.e., Aspergillus fumigatus, influenza viruses). PTX3 also regulates the clearance of apoptotic cells and may participate in maintenance of immunologic tolerance [13]. PTX3 is expressed in response to proinflammatory signals, including bacteria, IL-1 (but not IL-6), and TN-alpha produced primarily by endothelial cells, neutrophils, and macrophages. As a result, inflammation diseases, especially disorders of the immune system such as rheumatoid arthritis, progressive systemic sclerosis, Churg-Straus syndrome, Wegener’s granulomatosis, and microscopic polyangiitis, as well as systemic inflammatory response syndrome (SIRS), result in increased expression of plasma PTX3.
PTX3 is elevated in critically ill patients, with a gradient from systematic inflammatory response syndrome to septic shock, and in several other diseases, such as myocardial infarction, atherosclerosis, small vessel vasculitis and psoriasis. Plasma PTX3 levels have also been suggested to be a good marker for the response to treatment of patients with obstructive sleep apnea (OSA). PTX3 may also be a good diagnostic marker for deterioration in patients with inflammatory bowel disease.
Pentraxin 3 has been proposed as a marker of endothelial dysfunction and inflammation in pre-eclampsia. Pentraxin 3 levels have been found to be elevated in normal pregnancy and also shown to be significantly higher at the time of diagnosis of pre-eclampsia when compared with normal pregnancy. PTX3 may also be elevated in malignancies such as lung carcinoma, prostate cancer and liposarcomas.

References to Summary

References to Pentraxin 3

  • Alles VV, Bottazzi B, Peri G, Golay J, Introna M, Mantovani A. Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes. Blood. 1994 Nov 15;84 (10):3483-93
  • Baruah P, Propato A, Dumitriu IE, Rovere-Querini P, Russo V, Fontana R, Accapezzato D, Peri G, Mantovani A, Barnaba V, Manfredi AA. The pattern recognition receptor PTX3 is recruited at the synapse between dying and dendritic cells, and edits the cross-presentation of self, viral, and tumor antigens. Blood. 2006 Jan 1;107 (1):151-8
  • Bottazzi B, Vouret-Craviari V, Bastone A, De Gioia L, Matteucci C, Peri G, Spreafico F, Pausa M, D'Ettorre C, Gianazza E, Tagliabue A, Salmona M, Tedesco F, Introna M, Mantovani A. Multimer formation and ligand recognition by the long pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component. J Biol Chem. 1997 Dec 26;272 (52):32817-23
  • Breviario F, d'Aniello EM, Golay J, Peri G, Bottazzi B, Bairoch A, Saccone S, Marzella R, Predazzi V, Rocchi M, et al. Interleukin-1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component. J Biol Chem. 1992 Nov 5;267 (31):22190-7
  • Camozzi M, Zacchigna S, Rusnati M, Coltrini D, Ramirez-Correa G, Bottazzi B, Mantovani A, Giacca M, Presta M. Pentraxin 3 inhibits fibroblast growth factor 2-dependent activation of smooth muscle cells in vitro and neointima formation in vivo. Arterioscler Thromb Vasc Biol. 2005 Sep;25 (9):1837-42
  • Chen J, Xu X, Xia L, Xi X, Liu B, Yang M. Serum pentraxin 3 is a novel marker in Crohn's disease. Mol Med Rep. 2015 Jul;12 (1):543-6
  • Diamandis EP, Goodglick L, Planque C, Thornquist MD. Pentraxin-3 is a novel biomarker of lung carcinoma. Clin Cancer Res. 2011 Apr 15;17 (8):2395-9
  • Diniz SN, Nomizo R, Cisalpino PS, Teixeira MM, Brown GD, Mantovani A, Gordon S, Reis LF, Dias AA. PTX3 function as an opsonin for the dectin-1-dependent internalization of zymosan by macrophages. J Leukoc Biol. 2004 Apr;75 (4):649-56
  • Doni A, Michela M, Bottazzi B, Peri G, Valentino S, Polentarutti N, Garlanda C, Mantovani A. Regulation of PTX3, a key component of humoral innate immunity in human dendritic cells: stimulation by IL-10 and inhibition by IFN-gamma. J Leukoc Biol. 2006 Apr;79 (4):797-802
  • El Melegy EK, Badr EA, Elkersh AM, El Shafey WH, Fareed WA. Pentraxin 3 genotyping in relation to serum levels of pentraxin 3 in patients with acute ST-segment elevation myocardial infarction. Clinical Trials and Regulatory. 2016; (13):6-13
  • Fazzini F, Peri G, Doni A, Dell'Antonio G, Dal Cin E, Bozzolo E, D'Auria F, Praderio L, Ciboddo G, Sabbadini MG, Manfredi AA, Mantovani A, Querini PR. PTX3 in small-vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation. Arthritis Rheum. 2001 Dec;44 (12):2841-50
  • Fornai F, Carrizzo A, Forte M, Ambrosio M, Damato A, Ferrucci M, Biagioni F, Busceti C, Puca AA, Vecchione C. The inflammatory protein Pentraxin 3 in cardiovascular disease. Immun Ageing. 2016;13 (1):25
  • Garlanda C, Bottazzi B, Bastone A, Mantovani A. Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annu Rev Immunol. 2005;23:337-66
  • Garlanda C, Hirsch E, Bozza S, Salustri A, De Acetis M, Nota R, Maccagno A, Riva F, Bottazzi B, Peri G, Doni A, Vago L, Botto M, De Santis R, Carminati P, Siracusa G, Altruda F, Vecchi A, Romani L, Mantovani A. Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response. Nature. 2002 Nov 14;420 (6912):182-6
  • Grill S, Rusterholz C, Zanetti-Dallenbach R, Tercanli S, Holzgreve W, Hahn S, Lapaire O. Potential markers of preeclampsia--a review. Reprod Biol Endocrinol. 2009 Jul 14;7:70
  • Gumus P, Nizam N, Nalbantsoy A, Ozcaka O, Buduneli N. Saliva and serum levels of pentraxin-3 and interleukin-1beta in generalized aggressive or chronic periodontitis. J Periodontol. 2014 Mar;
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