Pig Unacylated Ghrelin EIA kit

Cat. No.	Size	Price
A05402	96 wells (1 kit)

Cat. No.

Size

Price

A05402

96 wells (1 kit)

Technical Data

Type

Sandwich ELISA, AChE-labelled antibody

Applications

Plasma

Sample Requirements

20 µl/well

Storage/Expiration

Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

3.9 – 500 pg/ml

Limit of Detection

The Limit of Determination, calculated as the concentration of Unacylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 5 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is 25 pg/mL.

Intra-assay (Within-Run)

CV = 8.2% (20h at +4°C) CV = 9.2% (3h at RT)

Inter-assay (Run-to-Run)

CV = 8.6% (20h at +4°C) CV = 9.5% (3h at RT)

Spiking Recovery

101.5% (20h at +4°C) , 99.8% (3h at RT)

Dilution Linearity

98,90%

Summary

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

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