Type
Recombinant protein
Description
Total 437 AA. MW: 47.8 kDa (calculated). UniProtKB acc.no. P59595. N-Terminal His-tag 16 AA.
Amino Acid Sequence
MRGSHHHHHHGMASHMSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA
Source
E. coli
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
12% SDS-PAGE separation of SARS-CoV Nucleocapsid protein
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane
Endotoxin
< 1.0 EU/µg
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M Acetate buffer pH= 4.0
Reconstitution
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited.
Applications
Western blotting
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Endotoxin level determination.
Note
This product is intended for research use only.
Research topic
Immune Response, Infection and Inflammation, COVID-19
Summary
Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The virus is a novel coronavirus isolated from patients exhibiting atypical pneumonia and may have originated from wild animals such as civet cats in southern China. The genome of SARS-CoV is a positive-sense, single-stranded RNA whose sequence is distantly related to all known coronaviruses that infect humans and animals. Like other known coronaviruses, SARS-CoV is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. The nucleocapsid (N) protein together with the viral RNA genome form a helical core located within the viral envelope.
The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV NP), a phosphoprotein of 48 kDa, is the most abundant protein in the virus-infected cells. Its primary function is to package the ∼30 kb single stranded, 5′-capped positive strand viral genome RNA molecule into a ribonucleoprotein (RNP) complex called the capsid. Ribonucleocapsid packaging is a fundamental part of viral self-assembly and the RNP complex constitutes the essential template for replication by the RNA-dependent RNA polymerase complex. In addition, the N-protein of the SARS-CoV has been shown to modulate the host cellular machinery and may serve regulatory roles during its viral life cycle.
As the most abundantly expressed structural protein during infection, SARS-CoV NP is highly detectable in SARS patients. Therefore, this protein may serve as one of the immunodominant antigens in the early diagnosis of infection. Furthermore, researchers have suggested that antibody against the N protein could modulate cytokine responses such as IL-11; non-neutralizing antibodies against N protein were found to protect mice against lethal infection.
Product Datasheet (RUO)
Datasheet PDF (RUO)
Datasheet PDF (RUO)
Safety Information (RUO)
MSDS (RUO)
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