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Manufactured by BioVendor

Proteinase 3 (PR3) Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Mouse
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Cat. No. Size Price


RAI002R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

2–8°C

Calibration Range

0.1 – 6.4 ng/ml

Summary

Research topic

Immune Response, Infection and Inflammation, Oncology, Animal studies

Summary

Proteinase 3 (PR3), also known as myeloblastin, Wegener autoantigen, PRTN3 and NP- 4, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs). The primary function of PR3 is recognized as to participate in direct intracellular killing of phagocytosed pathogens in phagolysosomes and degradation of extracellular matrix components at inflammatory sites. PR3 has also been proven to be able to process some pro-inflammatory cytokines, such as IL-1β, IL-18 and TNF-α, activate mitogen activated protein kinase (MAPK) signaling through proteinase activated receptor-1 (PAR1), and induce endothelial cell apoptosis through NF-κB signaling pathways. PR3 is identified as the target autoantigen of anti-neutrophil cytoplasmic autoantibodies (ANCA) in Wegener granulomatosis 6. Increased PR3 levels have been reported in patients with acute myocardial infarction, and in subjects with type 1 diabetes.

Product References (8)

References

  • Korkmaz B, Moreau T, Gauthier F. Neutrophil elastase, proteinase 3 andcathepsin G: physicochemical properties, activity and physiopathologicalfunctions. Biochimie. 2008 Feb;90(2):227-42. Epub 2007 Oct 25. Review. PubMedPMID: 18021746. See more on PubMed
  • Mihara K, Ramachandran R, Renaux B, Saifeddine M, Hollenberg MD. Neutrophilelastase and proteinase-3 trigger G protein-biased signaling throughproteinase-activated receptor-1 (PAR1). J Biol Chem. 2013 Nov15;288(46):32979-90. doi: 10.1074/jbc.M113.483123. Epub 2013 Sep 19. PubMed PMID:24052258; PubMed Central PMCID: PMC3829148. See more on PubMed
  • Nauseef WM, Borregaard N. Neutrophils at work. Nat Immunol. 2014Jul;15(7):602-11. doi: 10.1038/ni.2921. Review. PubMed PMID: 24940954. See more on PubMed
  • Ng LL, Khan SQ, Narayan H, Quinn P, Squire IB, Davies JE. Proteinase 3 andprognosis of patients with acute myocardial infarction. Clin Sci (Lond). 2011Mar;120(6):231-8. doi: 10.1042/CS20100366. PubMed PMID: 20942801; PubMed Central PMCID: PMC2999885. See more on PubMed
  • Pham CT. Neutrophil serine proteases: specific regulators of inflammation. NatRev Immunol. 2006 Jul;6(7):541-50. Review. PubMed PMID: 16799473. See more on PubMed
  • van der Geld YM, Limburg PC, Kallenberg CG. Proteinase 3, Wegener'sautoantigen: from gene to antigen. J Leukoc Biol. 2001 Feb;69(2):177-90. Review. PubMed PMID: 11272267. See more on PubMed
  • Wang Y, Xiao Y, Zhong L, Ye D, Zhang J, Tu Y, Bornstein SR, Zhou Z, Lam KS, XuA. Increased neutrophil elastase and proteinase 3 and augmented NETosis areclosely associated with β-cell autoimmunity in patients with type 1 diabetes.Diabetes. 2014 Dec;63(12):4239-48. doi: 10.2337/db14-0480. Epub 2014 Aug 4.PubMed PMID: 25092677. See more on PubMed
  • Wiedow O, Meyer-Hoffert U. Neutrophil serine proteases: potential keyregulators of cell signalling during inflammation. J Intern Med. 2005Apr;257(4):319-28. Review. PubMed PMID: 15788001. See more on PubMed
Summary References (8)

References to Proteinase-3

  • Korkmaz B, Moreau T, Gauthier F. Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions. Biochimie. 2008 Feb;90 (2):227-42
  • Mihara K, Ramachandran R, Renaux B, Saifeddine M, Hollenberg MD. Neutrophil elastase and proteinase-3 trigger G protein-biased signaling through proteinase-activated receptor-1 (PAR1). J Biol Chem. 2013 Nov 15;288 (46):32979-90
  • Nauseef WM, Borregaard N. Neutrophils at work. Nat Immunol. 2014 Jul;15 (7):602-11
  • Ng LL, Khan SQ, Narayan H, Quinn P, Squire IB, Davies JE. Proteinase 3 and prognosis of patients with acute myocardial infarction. Clin Sci (Lond). 2011 Mar;120 (6):231-8
  • Pham CT. Neutrophil serine proteases: specific regulators of inflammation. Nat Rev Immunol. 2006 Jul;6 (7):541-50
  • van der Geld YM, Limburg PC, Kallenberg CG. Proteinase 3, Wegener's autoantigen: from gene to antigen. J Leukoc Biol. 2001 Feb;69 (2):177-90
  • Wang Y, Xiao Y, Zhong L, Ye D, Zhang J, Tu Y, Bornstein SR, Zhou Z, Lam KS, Xu A. Increased Neutrophil Elastase and Proteinase 3 and Augmented NETosis Are Closely Associated with beta-cell Autoimmunity in Patients with Type 1 Diabetes. Diabetes. 2014 Aug 4;
  • Wiedow O, Meyer-Hoffert U. Neutrophil serine proteases: potential key regulators of cell signalling during inflammation. J Intern Med. 2005 Apr;257 (4):319-28
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