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Distributed product

PYY Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:Peptide YY
  • Species:Rat
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RSCYK081R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Plasma

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.15–12,5 ng/ml

Intra-assay (Within-Run)

CV = 3,1 - 9,8 %

Inter-assay (Run-to-Run)

CV = 4,2 - 14,2%

Summary

Research topic

Energy metabolism and body weight regulation, Animal studies

Summary

PYY was isolated initially by Tatemoto et al. (1980) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level decreases after resection of the intestine, possiblly be due to the decrease in number of the endocrine cells secreting PYY.

Product References (7)

References

  • Adrian TE, Smith HA, Calvert SA, Aynsley-Green A, Bloom SR. Elevated plasmapeptide YY in human neonates and infants. Pediatr Res. 1986 Dec;20(12):1225-7.PubMed PMID: 3797114. See more on PubMed
  • Adrian TE, Ferri GL, Bacarese-Hamilton AJ, Fuessl HS, Polak JM, Bloom SR.Human distribution and release of a putative new gut hormone, peptide YY.Gastroenterology. 1985 Nov;89(5):1070-7. PubMed PMID: 3840109. See more on PubMed
  • El-Salhy M, Grimelius L, Wilander E, Ryberg B, Terenius L, Lundberg JM,Tatemoto K. Immunocytochemical identification of polypeptide YY (PYY) cells inthe human gastrointestinal tract. Histochemistry. 1983;77(1):15-23. PubMed PMID: 6341321. See more on PubMed
  • Greeley GH Jr, Hashimoto T, Izukura M, Gomez G, Jeng J, Hill FL, Lluis F,Thompson JC. A comparison of intraduodenally and intracolonically administerednutrients on the release of peptide-YY in the dog. Endocrinology. 1989Oct;125(4):1761-5. PubMed PMID: 2791964. See more on PubMed
  • Greeley GH Jr, Hill FL, Spannagel A, Thompson JC. Distribution of peptide YYin the gastrointestinal tract of the rat, dog, and monkey. Regul Pept. 1987Dec;19(5-6):365-72. PubMed PMID: 3438492. See more on PubMed
  • Gomez G, Zhang T, Rajaraman S, Thakore KN, Yanaihara N, Townsend CM Jr,Thompson JC, Greeley GH. Intestinal peptide YY: ontogeny of gene expression inrat bowel and trophic actions on rat and mouse bowel. Am J Physiol. 1995Jan;268(1 Pt 1):G71-81. PubMed PMID: 7840209. See more on PubMed
  • Larhammar D. Evolution of neuropeptide Y, peptide YY and pancreaticpolypeptide. Regul Pept. 1996 Apr 9;62(1):1-11. Review. PubMed PMID: 8738876. See more on PubMed
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