Competitive ELISA, Immobilized antigen
RatLaps™ (CTX-I) EIA is an enzyme-linked immunosorbent assay for the quantitative determination of bone-related degradation products from C-terminal telopeptides of type I collagen in rat/mouse serum or urine, and from rat/mouse bone released into cell culture supernatants by osteoclasts. The assay is for research use only.
Type I collagen accounts for more than 90% of the organic matrix of bone and is synthesised primary in bone. During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation. The RatLaps™ (CTX-I) EIA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption. The RatLaps™ (CTX-I) EIA is based upon the competitive binding of a polyclonal antibody to soluble RatLaps antigens EKSQDGGR or to immobilised RatLaps antigens. Briefly, the polyclonal antibody is raised against a synthetic peptide having a sequence (EKSQDGGR) specific for a part of the C-terminal telopeptide α1 chain of rat type I collagen. For standardisation of the RatLaps™ (CTX-I) EIA a synthetic peptide (EKSQDGGR) that is specific for the C-terminal telopeptide α1 chain of type I collagen in rats has been used.
Serum, Urine, Plasma, Cell culture supernatant
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection