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sCD44std Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Phagocytic glycoprotein I, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, CD44 antigen
  • Species:Human
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Cat. No. Size Price


RAF095R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine, Amniotic fluid, Plasma, Cell culture supernatant

Sample Requirements

20 µl (1:60 prediluted)

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.12–4 ng/ml

Limit of Detection

0.015 ng/ml

Intra-assay (Within-Run)

CV = 4.8%

Inter-assay (Run-to-Run)

CV = 4.1%

Spiking Recovery

89,00%

Dilutation Linearity

94,00%

Summary

Research topic

Cell adhesion proteins, Cell surface proteins (sCD), Oncology

Summary

CD44 (85-250 kDa) is a polymorphic glycoprotein.
Determination of sCD44std will provide more detailed insight into different pathological modifications during cancer and other diseases.

  • brain tumors: CD44 is strongly expressed in high-grade gliomas and weakly expressed in meningiomas, medulloblastomas and normal brain.
  • colorectal carcinomas: in human colorectal neoplasia CD44 variant proteins are found on all invasive carcinomas and during carcinoma metastasis. Variants are already expressed at a relatively early stage of colorectal carcinogenesis and tumor progression.
  • gastric cancer: tumors from patients suffering from stomach adenocarcinomas express CD44 variants. Adenocarcinomas of the intestinal type are strongly positive for exon v5 and v6, whereas diffuse type adenocarcinomas predominantly express exon v5.
  • lung, breast cancer: in malignant tissues there is gross overproduction of alternatively-spliced large molecular variants in all samples, whereas in the control samples only the standard product was routinely detected with occasional minimal quantities of one or two small variants.
  • lymphoma: in gastrointestinal lymphoma overexpression of CD44 has been correlated with poor survival and more disseminated disease (6,3). Overexpression of CD44 is also found in several aggressive, but not low-grade, non-Hodgkin´s lymphomas (7) as well as in Hodgkin´s and nodal diffuse lymphomas.
  • tonsil, skin cancer: variant CD44 isoform expression can be demonstrated in the plasma membrane of squamous cells of skin and tonsil epithelial and is greatly diminished in malignant squamous epithelial tumors.
  • HIV: CD44 is almost completely depleted from the surface of HIV-infected cells.
  • inflammatory joint diseases: CD44 expression was decreased in synovial fluid neutrophils from most patients.
References to Summary

References to CD44

  • Arch R, Wirth K, Hofmann M, Ponta H, Matzku S, Herrlich P, Zoller M. Participation in normal immune responses of a metastasis-inducing splice variant of CD44. Science. 1992 Jul 31;257 (5070):682-5
  • Fujiwara K, Yoshino T, Miyake K, Ohara N, Akagi T. Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage. Acta Med Okayama. 1993 Jun;47 (3):215-22
  • Gunthert U, Hofmann M, Rudy W, Reber S, Zoller M, Haussmann I, Matzku S, Wenzel A, Ponta H, Herrlich P. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell. 1991 Apr 5;65 (1):13-24
  • Guo MM, Hildreth JE. HIV-induced loss of CD44 expression in monocytic cell lines. J Immunol. 1993 Aug 15;151 (4):2225-36
  • Heider KH, Dammrich J, Skroch-Angel P, Muller-Hermelink HK, Vollmers HP, Herrlich P, Ponta H. Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa. Cancer Res. 1993 Sep 15;53 (18):4197-203
  • Heider KH, Hofmann M, Hors E, van den Berg F, Ponta H, Herrlich P, Pals ST. A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps. J Cell Biol. 1993 Jan;120 (1):227-33
  • Hofmann M, Rudy W, Zoller M, Tolg C, Ponta H, Herrlich P, Gunthert U. CD44 splice variants confer metastatic behavior in rats: homologous sequences are expressed in human tumor cell lines. Cancer Res. 1991 Oct 1;51 (19):5292-7
  • Humbria A, Diaz-Gonzalez F, Campanero MR, Arroyo AG, Laffon A, Gonzalez-Amaro R, Sanchez-Madrid F. Expression of L-selectin, CD43, and CD44 in synovial fluid neutrophils from patients with inflammatory joint diseases. Evidence for a soluble form of L-selectin in synovial fluid. Arthritis Rheum. 1994 Mar;37 (3):342-8
  • Joensuu H, Ristamaki R, Klemi PJ, Jalkanen S. Lymphocyte homing receptor (CD44) expression is associated with poor prognosis in gastrointestinal lymphoma. Br J Cancer. 1993 Aug;68 (2):428-32
  • Koopman G, Heider KH, Horst E, Adolf GR, van den Berg F, Ponta H, Herrlich P, Pals ST. Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J Exp Med. 1993 Apr 1;177 (4):897-904
  • Kuppner MC, Van Meir E, Gauthier T, Hamou MF, de Tribolet N. Differential expression of the CD44 molecule in human brain tumours. Int J Cancer. 1992 Feb 20;50 (4):572-7
  • Matsumura Y, Tarin D. Significance of CD44 gene products for cancer diagnosis and disease evaluation. Lancet. 1992 Oct 31;340 (8827):1053-8
  • Moller P, Eichelmann A, Mechtersheimer G, Koretz K. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node. Int J Cancer. 1991 Dec 2;49 (6):846-55
  • Omary MB, Trowbridge IS, Letarte M, Kagnoff MF, Isacke CM. Structural heterogeneity of human Pgp-1 and its relationship with p85. Immunogenetics. 1988;27 (6):460-4
  • Salmi M, Gron-Virta K, Sointu P, Grenman R, Kalimo H, Jalkanen S. Regulated expression of exon v6 containing isoforms of CD44 in man: downregulation during malignant transformation of tumors of squamocellular origin. J Cell Biol. 1993 Jul;122 (2):431-42
  • Stamenkovic I, Aruffo A, Amiot M, Seed B. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J. 1991 Feb;10 (2):343-8
  • Underhill C. CD44: the hyaluronan receptor. J Cell Sci. 1992 Oct;103 ( Pt 2):293-8
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