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sL-Selectin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1, Lymph node homing receptor, Lymphocyte adhesion molecule 1, CD62L
  • Species:Human
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Cat. No. Size Price


RAF108R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine, Cell culture supernatant

Sample Requirements

50 µl (1:100 prediluted)

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.39–25 ng/ml

Limit of Detection

0.19 ng/ml

Intra-assay (Within-Run)

CV = 3.7%

Inter-assay (Run-to-Run)

CV = 4.2%

Spiking Recovery

99,00%

Dilutation Linearity

89,10%

Summary

Research topic

Cell adhesion proteins, Oncology

Summary

Determination of soluble/circulating L-selectin could provide more detailed insights into the pathological modifications during various diseases:

  • allergy: L-selectin expression is down-modulated on eosinophils recovered from bronchoalveolar lavage fluid after allergen provocation.
  • bronchoalveolar lavage (BAL): BAL transiently promotes PMN/monocyte activation and recruitment to the bronchoalveolar space. The cells respond with a complete shedding of L-selectin when they extravasate from the blood into the bronchoalveolar space.
  • deep venous thrombosis (DVT): A case can be made for the participation of PMN's in the initiation and propagation of venous thrombosis. Probably via L-selectin leukocytes adhere to areas of veins that serve as sites for initiation of thrombi.
  • HIV: patients suffering from HIV-infection showed markedly elevated levels of sL-selectin in serum.
  • insulin-dependent diabetes mellitus (IDDM): serum levels of L-selectin were found to be elevated in IDDM patients and in subjects at risk for developing IDDM.
  • Kawasaki Syndrome: sL-selectin levels seem to be less than those of normals.
  • malignant B-cell populations: B-cell chronic lymphocytic leukaemia, hairy cell leukaemia and mantle zone lymphoma are L-selectin positive.
  • neonatal bacterial infection: in case of intra-uterine infection lymphocytes obtained from cord blood have a diminished L-selectin expression. This is independent of gestational age, birth weight, umbilical artery pH, hematocrit, leukocyte count, absolute neutrophil count, CRP-level or maternal fever .
  • sepsis: patients suffering from sepsis showed markedly elevated levels of sL-selectin in serum. Vascular endothelial injury observed in overwhelming sepsis may be caused by neutrophil-derived enzymes. Adherence to endothelium is a prerequisite for this process. Measurement of sL-selectin may provide further insights into the interrelationship between neutrophil activation and endothelial damage in gram-negative sepsis.
  • surgery: patients undergoing cardiopulmonary bypass surgery may develop an acute post-operative capillary leak, due to endothelial injury inflicted by adherent neutrophils. In those patients L-selectin is completely lost in a small but progressively increasing proportion of PMN's, which could be responsible for the endothelial damage.
References to Product

References

  • Kotyla PJ. Short course of simvastatin has no effect on markers of endothelial activation in normolipidemic patients with systemic sclerosis. J Int Med Res. 2018;46(5):1893-1901.
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