Select country set
Menu Shopping cart 0,00 Search
Manufactured by BioVendor

sPLA2-IIA Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Phospholipase A2 membrane associated, GIIC sPLA2, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2, NPS-PLA2, Phosphatidylcholine 2-acylhydrolase 2A, PLA2G2A, PLA2B, PLA2L, RASF-A
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price

RD172054100 0.1 mg
PubMed Product Details
Technical Data


Recombinant protein


Total 140 AA. MW: 15.8 kDa (calculated). UniProtKB P14555. N-Terminal His-tag, 16 extra AA (highlighted).

Amino Acid Sequence



E. coli


Purity as determined by densitometric image analysis: >95%


12% SDS-PAGE separation of Human sPLA2-IIA
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 10μg/lane
3. non-reduced and non-heated sample, 10μg/lane


< 1.0 EU/ug


Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH4


Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.


Western blotting


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.


This product is intended for research use only.

Product References (4)


  • Jayaraman S, Fändrich M, Gursky O. Synergy between serum amyloid A and secretory phospholipase A(2). Elife. 2019 May 21;8:e46630. doi: 10.7554/eLife.46630. PubMed PMID: 31111824. PubMed CentralPMCID: PMC6557629. See more on PubMed
  • Lu S, Dong Z. Overexpression of secretory phospholipase A2-IIa supports cancer stem cell phenotype via HER/ERBB-elicited signaling in lung and prostate cancer cells. Int J Oncol. 2017 Jun;50(6):2113-2122. doi: 10.3892/ijo.2017.3964. Epub 2017 Apr 19. PubMed PMID: 28440478. See more on PubMed
  • Tanabe T, Shimokawaji T, Kanoh S, Rubin BK. Secretory phospholipases A2 are secreted from ciliated cells and increase mucin and eicosanoid secretion from goblet cells. Chest. 2015 Jun;147(6):1599-1609. doi: 10.1378/chest.14-0258. PubMed PMID: 25429648. PubMed CentralPMCID: PMC4451714. See more on PubMed
  • Rönkkö S, Rekonen P, Kaarniranta K, Puustjarvi T, Teräsvirta M, Uusitalo H. Phospholipase A2 in chamber angle of normal eyes and patients with primary open angle glaucoma and exfoliation glaucoma. Mol Vis. 2007 Mar 26;13:408-17. PubMed PMID: 17417602. PubMed CentralPMCID: PMC2642936. See more on PubMed
Related Products Docs