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Manufactured by BioVendor

Taq Plus DNA Polymerase

  • Regulatory status:RUO
  • Type:Recombinant protein
Cat. No. Size Price


New RP179309250 250 U $145
New RP1793092500 2,500 U $495
New RP179309750 750 U $295
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Stable for 5 days at 10°C, for longer period of time store at -20°C.

Summary

Summary

Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74ºC and exhibits a half-life of 40 minutes at 95ºC. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

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