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Human Thyroxine, Total (T4) ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD026R 96 wells (1 kit) $358,83
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the direct quantitative determination of Thyroxine by enzyme immunoassay in human serum. For research use only.



Sample Requirements

20 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1–32 µg%

Limit of Detection

0.6 µg%

Intra-assay (Within-Run)


Inter-assay (Run-to-Run)




  • RUO
  • calibration range 1-32 µg%
  • limit of detection 0.6 µg%
  • intra-assay CV = 7.5%
  • inter-assay CV = 11.2%
  • controls

Research topic



Clinical Trends: − The level of T4 in hypothyroid patients is decreased. − The level of T4 in hyperthyroid patients is increased. − In euthyroid individuals the level of T4 is within the normal range. Thyroid binding globulin (TBG) levels have reportedly been elevated by the following conditions: increased estrogens from oral contraceptives, androgens, glucocorticords and pregnancy. Consequently, borderline T4 values should be viewed with caution when any of the above conditions are present.

Summary References (8)

References to Thyroxine

  • Comoglio S, Celada F. An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label. J Immunol Methods. 1976 Mar;10 (2-3):161-70
  • Ingbar SH, Braverman LE, Dawber NA, Lee GY. A new method for measuring the free thyroid hormone in human serum and an analysis of the factors that influence its concentration. J Clin Invest. 1965 Oct;44 (10):1679-89
  • McComb RB, Bowers GN, Posen S. Alkaline Phosphatase. Plenum Press, New York. 1979;1st Ed., Chap. 9:525-704
  • Robbins J. Radioassay and the thyroid gland. Metabolism. 1973 Aug;22 (8):1021-6
  • Schall RF Jr, Fraser AS, Hansen HW, Kern CW, Tenoso HJ. A sensitive manual enzyme immunoassay for thyroxine. Clin Chem. 1978 Oct;24 (10):1801-4
  • Selenkow HA, Robin NI. The diagnosis and management of common thyroid diseases. J Maine Med Assoc. 1970 Oct;61 (10):199-211 passim
  • Sterling K, Hegedus A. Measurement of free thyroxine concentration in human serum. J Clin Invest. 1962 May;41:1031-40
  • Surks MI, Squef R, Oppenheimer JH, Hauer H. Binding of Thyroxine by serum proteins evaluated by equilibrum dialysis and electrophoretic techniques. Alterations in nonthyroidal illness. J Clin Invest.. 1963 Nov; 42 :1769-82
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