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17-OH-Progesterone free in saliva ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:17α-OHP
  • Species:Multispecies
Cat. No. Size Price

RTC010R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

25 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

10 – 1000 pg/ml

Limit of Detection

2.5 pg/ml



Incubation time:60 min, 15 min at RT

Research topic

Steroid hormones, Animal studies


The steroid 17α−OH Progesterone is produced by the adrenal cortex and gonads. The 17α−OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21-hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21– hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17αOHP is secreted in abundant excess. Measurment of 17αOHP is therefore valuable in the initial diagnosis of CAH.

Summary References (4)

References to 17α-Hydroxyprogesterone

  • Hofman LF, Klaniecki JE, Smith EK. Direct solid-phase radioimmunoassay for screening 17 alpha-hydroxyprogesterone in whole-blood samples from newborns. Clin Chem. 1985 Jul;31 (7):1127-30
  • Sippell WG, Dorr HG, Bidlingmaier F, Knorr D. Plasma levels of aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-hydroxyprogesterone, cortisol, and cortisone during infancy and childhood. Pediatr Res. 1980 Jan;14 (1):39-46
  • Thorneycroft IH, Mishell DR Jr, Stone SC, Kharma KM, Nakamura RM. The relation of serum 17-hydroxyprogesterone and estradiol-17-beta levels during the human menstrual cycle. Am J Obstet Gynecol. 1971 Dec 1;111 (7):947-51
  • Yeo KH, Elder PA, Lewis JG. An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate. Ann Clin Biochem. 1988 May;25 ( Pt 3):293-7
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