Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum
Sample Requirements
50 μl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
100-0,3 u/ml
Limit of Detection
0,13 u/ml
Intra-assay (Within-Run)
4,8 %
Inter-assay (Run-to-Run)
6,2 %
Research topic
Autoimmunity
Summary
Autoimmune destruction of the adrenal cortex is the most common cause of Addison’s disease and autoantibodies to the adrenal specific enzyme steroid 21-OH hydroxylase are important markers of adrenal autoimmunity. This can be the case if the disease presents as Addison’s disease or as part of the autoimmune polyglandular syndromes (APS) type I or type II.
References
-E. S. Husebye et al Consensus Statement on the Diagnosis, Treatment and Follow-up of Patients with Primary Adrenal Insufficiency J Intern Med. 2014 275(2):104-15
- G. Coco et al Estimated Risk for Developing Autoimmune Addison’s Disease in Patients with Adrenal Cortex Autoantibodies J. Clin. Endocrinol. Metab. 2006 91: 1637-1645
- H. Tanaka et al Steroid 21-Hydroxylase Autoantibodies: Measurements with a New Immunoprecipitation Assay
J. Clin. Endocrinol. Metab. 1997 82: 1440-1446
- J. Furmaniak and B. Rees Smith Editorial: Adrenal and Gonadal Autoimmune Diseases J. Clin. Endocrinol. Metab. 1995 80: 1502 – 1505
- S. Chen et al Autoantibodies to Steroidogenic Enzymes in Autoimmune Polyglandular Syndrome, Addison’s Disease, and Premature Ovarian Failure J. Clin. Endocrinol. Metab. 1996 81: 1871-1876
