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25-OH-Vitamin-D ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
This product is not available in United States!
Cat. No. Size Price


EA300/96 96-wells
PubMed Product Details
Technical Data

Cat # changed from REA300/96 to EA300/96

Type

Competitive ELISA, Immobilized antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µL/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

4 – 120 ng/ml

Limit of Detection

1.6 ng/ml

Intra-assay (Within-Run)

n = 40; CV = 5.0 %

Inter-assay (Run-to-Run)

n = 10; CV = 7.8 %

Summary

Research topic

Bone and cartilage metabolism

Summary

Vitamin D is a steroid hormone involved in the intestinal absorption of calcium and the regulation of calcium homeostasis. There are two different forms of Vitamin D, named D3 and D2, which are very similar in structure. The D2 is a synthetic product, which is predominantly absorbed by fortified food. Physiological Vitamin D3 levels result not only from dietary uptake but also by biosynthesis of 7-dehydrocholesterol and UV-light in skin because of sun exposure. Vitamin D deficiency can result from inadequate intake coupled with inadequate sunlight exposure, disorders that limit its absorption, conditions that impair conversion of vitamin D into active metabolites, such as liver or kidney disorders, or, rarely, by a number of hereditary disorders. Deficiency results in impaired bone mineralization, and leads to bone softening diseases, rickets in children and osteomalacia in adults, and possibly contributes to osteoporosis. Research has indicated that vitamin D deficiency is linked to colon cancer; conflicting evidence links vitamin D deficiency to other forms of cancer. In the liver, the vitamin is hydroxylated to 25-hydroxyvitamin D (25-OH Vitamin D), the major circulating metabolite of Vitamin D. Although 1,25-(OH)2 Vitamin D portrays the biological active form of Vitamin D, which is synthesized in the kidney, it is widely accepted that the measurement of circulating 25-OH Vitamin D provides better information with respect to patients Vitamin D status and allows its use in diagnose hypovitaminosis . The concentration of 25-OH Vitamin D decreases with age and a deficiency is common among elderly persons. Clinical applications of 25-OH Vitamin D measurements are the diagnosis and therapy control of postmenopausal osteoporosis, rickets, osteomalacia, renal osteodystrophy, pregnancy, neonatal hypocalcemia and hyperparathyro­idism. Vitamin D intoxication mostly occurs during a large intake of pharmaceutical preparations of Vitamin D and may lead to hypercalcemia, hypercalcuria and nephrocalcinosis in susceptible infants.

Product References (5)

References

  • Mumford SL, Garbose RA, Kim K, Kissell K, Kuhr DL, Omosigho UR, Perkins NJ, Galai N, Silver RM, Sjaarda LA, Plowden TC, Schisterman EF. Association of preconception serum 25-hydroxyvitamin D concentrations with livebirth and pregnancy loss: a prospective cohort study. Lancet Diabetes Endocrinol. 2018 Sep;6(9):725-732. doi: 10.1016/S2213-8587(18)30153-0. Epub 2018 May 31. PubMed PMID: 29859909. PubMed CentralPMCID: PMC6109429. See more on PubMed
  • Kuhr DL, Sjaarda LA, Alkhalaf Z, Omosigho UR, Connell MT, Silver RM, Kim K, Perkins NJ, Holland TL, Plowden TC, Schisterman EF, Mumford SL. Vitamin D is associated with bioavailability of androgens in eumenorrheic women with prior pregnancy loss. Am J Obstet Gynecol. 2018 Jun;218(6):608.e1-608.e6. doi: 10.1016/j.ajog.2018.03.012. Epub 2018 Mar 13. PubMed PMID: 29548752. PubMed CentralPMCID: PMC6191853. See more on PubMed
  • Parfieniuk-Kowerda A, Świderska M, Rogalska M, Maciaszek M, Jaroszewicz J, Flisiak R. Chronic hepatitis B virus infection is associated with decreased serum 25(OH)D concentration in non-cirrhotic patients. Clin Exp Hepatol. 2019 Mar;5(1):75-80. doi: 10.5114/ceh.2019.83160. Epub 2019 Feb 20. PubMed PMID: 30915410. PubMed CentralPMCID: PMC6431090. See more on PubMed
  • Ciebiera M, Włodarczyk M, Słabuszewska-Jóźwiak A, Ciebiera M, Nowicka G, Jakiel G. Vitamin D serum levels in women using contraception containing drospirenone - a preliminary study. Arch Med Sci. 2019 Mar;15(2):554-557. doi: 10.5114/aoms.2017.70887. Epub 2017 Oct 20. PubMed PMID: 30899309. PubMed CentralPMCID: PMC6425194. See more on PubMed
  • Jahn D, Dorbath D, Kircher S, Nier A, Bergheim I, Lenaerts K, Hermanns HM, Geier A. Beneficial Effects of Vitamin D Treatment in an Obese Mouse Model of Non-Alcoholic Steatohepatitis. Nutrients. 2019 Jan 3;11(1):77. doi: 10.3390/nu11010077. PubMed PMID: 30609782. PubMed CentralPMCID: PMC6356425. See more on PubMed
Summary References (7)

References to 25-OH-Vitamin-D

  • Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with healthconsequences. Am J Clin Nutr. 2008 Apr;87(4):1080S-6S. Review. PubMed PMID:18400738. See more on PubMed
  • Hollis BW. Editorial: The determination of circulating 25-hydroxyvitamin D: noeasy task. J Clin Endocrinol Metab. 2004 Jul;89(7):3149-51. PubMed PMID:15240585. See more on PubMed
  • Lips P. Vitamin D physiology. Prog Biophys Mol Biol. 2006 Sep;92(1):4-8. Epub 2006 Feb 28. Review. PubMed PMID: 16563471. See more on PubMed
  • Macdonald HM, Mavroeidi A, Fraser WD, Darling AL, Black AJ, Aucott L, O'Neill F, Hart K, Berry JL, Lanham-New SA, Reid DM. Sunlight and dietary contributionsto the seasonal vitamin D status of cohorts of healthy postmenopausal womenliving at northerly latitudes: a major cause for concern? Osteoporos Int. 2011Sep;22(9):2461-72. doi: 10.1007/s00198-010-1467-z. Epub 2010 Nov 18. Erratum in: Osteoporos Int. 2011 Sep;22(9):2473-4. PubMed PMID: 21085934. See more on PubMed
  • Mavroeidi A, O'Neill F, Lee PA, Darling AL, Fraser WD, Berry JL, Lee WT, Reid DM, Lanham-New SA, Macdonald HM. Seasonal 25-hydroxyvitamin D changes in British postmenopausal women at 57 degrees N and 51 degrees N: a longitudinal study. JSteroid Biochem Mol Biol. 2010 Jul;121(1-2):459-61. doi:10.1016/j.jsbmb.2010.03.038. Epub 2010 Mar 17. PubMed PMID: 20302933. See more on PubMed
  • Snellman G, Melhus H, Gedeborg R, Byberg L, Berglund L, Wernroth L,Michaëlsson K. Determining vitamin D status: a comparison between commerciallyavailable assays. PLoS One. 2010 Jul 13;5(7):e11555. doi:10.1371/journal.pone.0011555. Erratum in: PLoS One. 2010;5(9). doi:10.1371/annotation/23307aa4-726e-4f11-86c0-8a292be33517. PubMed PMID: 20644628;PubMed Central PMCID: PMC2903481. See more on PubMed
  • Tsur A, Metzger M, Dresner-Pollak R. Effect of different dress style onvitamin D level in healthy young Orthodox and ultra-Orthodox students in Israel. Osteoporos Int. 2011 Nov;22(11):2895-8. doi: 10.1007/s00198-010-1492-y. Epub 2010Nov 26. PubMed PMID: 21110005. See more on PubMed
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