About

Coherence-controlled holographic microscopy

Raman spectroscopy is based on the Raman effect: when a molecule is exposed to laser light, a small fraction is scattered with a shift in frequency compared to the incident light. This shift is highly specific for each molecule – as unique as a fingerprint.

Principles of quantitative phase imaging

The time of propagation of light in a specific environment depends on the refractive index as well as the distance of the optical path. Therefore, when a light wave travels through a sample with varying refractive index and/or height, its wavefront is distorted and a change in the phase distribution of this wave occurs. The Q-PHASE is able to detect the phase distribution in the sample plane. This process of phase detection in a sample plane is usually referred to as quantitative phase imaging. Quantitative phase image can provide information on sample morphology, topography or cell dry-mass distribution. [5,6] Cell dry mass is quantified in pg/μm2 and can be calculated directly from phase values detected in each pixel. Quantitative phase imaging provides a very simple and sensitive way for monitoring of cell reactions to treatment and analyses of movement, growth, area, shape and many other parameters. Various color LUTs are often used for representation of phase images to easily distinguish different phase values.

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Patented optical setup

The Q-PHASE microscope consists of two arms, object arm and reference arm. The arms have similar microscope setups with a common illumination system. The sample is placed into the object arm, and the so-called reference sample (blank) is placed into the reference arm. The beams in each arm pass through the inserted sample and are combined at the image plane of the microscope. Thanks to the Q-PHASE’s unique patented optical setup, the beams interfere and form a hologram even when illuminated with a halogen lamp or a LED. The hologram is then recorded by a detector and a quantitative phase image is extracted from the hologram in real time by a computer.

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microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
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Format of Panel

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Biofluid miRNA knowledge panel

CAT. NO.	Name	DESCRIPTION	SAMPLES
1105151	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 2 x 96-well PCR plates	2
1105152	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 6 x 96-well PCR plates	6
1105153	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates	12
1105154	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	24
1105251	ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (2)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 4 x 96-well PCR plates	2
1105252	ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates	6
1105253	ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	12
1105254	ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24)	4 x 24 μl of ID3EAL miRNA RT Primers 96-plex 48 x 96-well PCR plates	24

Choose your thermal cycler

Roche
Abi Fast
Abi Standard
Bio-Rad

Biofluid miRNA Panel Analysis Template

ID3EAL Cancer miRNA Panel

ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

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Number of miRNAs Detected CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.

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Format of Panel

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Cancer miRNA knowledge panel

CAT. NO.	Name	DESCRIPTION	SAMPLES
1105151	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 2 x 96-well PCR plates	2
1105152	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 6 x 96-well PCR plates	6
1105153	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12)	1 x 24 μl of ID3EAL miRNA RT Primers 96-plex 12 x 96-well PCR plates	12
1105154	ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	24
1105261	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (2)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 8 x 96-well PCR plates	2
1105262	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 24 x 96-well PCR plates	6
1105263	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 48 x 96-well PCR plates	12
1105264	ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24)	2 x 24 μl of ID3EAL miRNA RT Primers 96-plex 96 x 96-well PCR plates	24

Choose your thermal cycler

Roche
Abi Fast
Abi Standard
Bio-Rad

Cancer miRNA Panel Analysis Template