Type
Sandwich ELISA, Biotin-labelled antibody
Description
BioVendor’s AChRAb ELISA depends on the ability of AChRAb in human serum to bind to similar sites on the AChR as various monoclonal antibodies such as MAb1 (coated on ELISA plate wells) and/or MAb2 and/or MAb3 (which are labelled with Biotin).
Applications
Serum
Sample Requirements
100 μl
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.5–20 nmol/l
Limit of Detection
0.25 nmol/l
Intra-assay (Within-Run)
CV = 5.4%
Inter-assay (Run-to-Run)
CV = 9,7%
Specificity
Sera from 302 individual healthy blood donors were assayed in the AChRAb ELISA. 401 (99.8%) were identified as being negative for AChRAb. One sample was positive and gave a value of 20% inhibition (0.54 nmol/L from the calibration curve, 0.52 nmol/L calculated).