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Advantages

  • No image artifacts such as halo effect (as opposed to techniques based on Zernike phase contrast illumination)
  • Enables very precise detection of cell boundaries
  • Strong suppression of coherent noise (speckles) & parasitic interferences
  • (as opposed to laser-based approaches)
  • Label-free – no staining is needed, simple sample preparation, observation
  • of live cells in their native environment, no photobleaching problems
  • Low phototoxicity – low light power density (107× lower than fluorescence
  • microscopy) allows long-term observations (for days)
  • Coherence-gating effect – Q-PHASE special feature enables it to observe samples
  • even in scattering media (phospholipid emulsions, extracellular matrices, etc.)
  • Multimodality – fully integrated fluorescence module, simulated DIC and
  • brightfield which enables automatic multimodal imaging of the sample
  • High-quality QPI – unique Q-PHASE’s optical setup allows using incoherent illumination
  • which provides extraordinary imaging quality without any compromises
  • Lateral resolution of conventional microscopes (up to 2× better when compared to common laser-based approaches or pinhole spatial filtering based techniques)
  • Fast acquisition – the use of off-axis holographic approach makes Q-PHASE a single-shot instrument, thus enabling imaging of very fast cell dynamics
  • Full motorization – focusing, sample stage, objective exchange, fluorescence
  • filters
  • Automated multidimensional acquisition – time-lapse, channel, position, Z-stack
  • Simple image segmentation and processing – comparable to fluorescence data processing
  • Quantitative – phase values can be recalculated (e.g. to cell dry-mass density (pg/μm2) or direct topography) with nanometer sensitivity (usually non-biological samples with homogeneous refractive index distribution)
  • High phase detection sensitivity – is able to detect even the smallest changes in axial direction, very sensitive detection of morphology or position changes

Fluorescence module

The Q-PHASE can combine holographic microscopy with fluorescence microscopy. This powerful combination provides the possibility to verify structures or processes observed in QPI with fluorescence microscopy in the same field of view using a single instrument. For example, morphological and position changes prior to cell death can be observed in QPI followed by fluorescence verification of cell death types (see images below) [4]. This approach greatly reduces the phototoxicity and photobleaching problems of fluorescence imaging and it allows long-term observations.

The focus plane in both methods is located at the same position. This allows easy and fast switching between the two imaging methods at the same conditions and time points. Multiple fluorescence channels are possible with motorized channel exchange for automated multidimensional measurements.

The illumination can be implemented by using liquid light guide coupled solid state light sources or a xenon arc lamp. Multidimensional image acquisition combining holography and fluorescence is fully integrated in the Q-PHASE’s software. A fluorescence module is attached to the side port of the Q-PHASE, which can alternatively be used for other imaging techniques.

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Intrinsic imaging modes

Complementary image contrast can be obtained simply by numerical processing of the acquired phase images. In this way simulated DIC images can be produced with adjustable shear and displayed in real time. Another possibility is brightfield imaging which can be simply achieved by closing the reference arm of the microscope. In summary, the Q-PHASE offers multiple imaging modes widely used in biological research such as fluorescence or DIC integrated in a single instrument and supported by the Q-PHASE’s software allowing fully automated multimodal imaging.

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Imaging in scattering media

A special feature of Q-PHASE is coherence-gating, a well-known effect in optical coherence tomography which enables observations of samples even in scattering media. This effect is induced by using incoherent light in the unique patented setup of Q-PHASE. Its transmitted-light configuration enables it to effectively suppress the light which was scattered by the environment in defocused planes and to only use unscattered light for imaging. In this way, cells can be observed even in moderately scattering non-transparent substances such as an active phospholipid emulsion.

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Imaging in extracellular matrices

The coherence-gating effect can also be beneficial when imaging cells in extracellular matrices such as collagen gel. Extracellular matrices mimic an in vivo environment making the study of the cell’s dynamic reactions to its surroundings more realistic. Usually it is used as a biological test for cancer cell invasivity and ability to metastasize. The Q-PHASE microscope enables one to record a mechanism of cell motion and interactions between extracellular matrix fibers and cells with high contrast and without any additional staining.

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microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

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Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
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Format of Panel

 

11051 

Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
24

 

For Purchase Calculation please download Knowledge Panel calculator here:

Knowledge Panel calculator

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template 

 

ID3EAL Cancer miRNA Panel

ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

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Number of miRNAs Detected                                                  CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.


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Format of Panel

11051

 Cancer miRNA knowledge panel 

CAT. NO. Name DESCRIPTION SAMPLES
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105262 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
6
1105263 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
12
1105264 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
96 x 96-well PCR plates
24


For Purchase Calculation please download Knowledge Panel calculator here:

Knowledge Panel calculator

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Cancer miRNA Panel Analysis Template