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Albumin Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Mouse
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RSHAKRAL121R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine, Plasma, Cell culture supernatant

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

50–1000 ng/ml

Limit of Detection

2.5 ng/ml

Intra-assay (Within-Run)

n = 5; CV = 5.0%

Inter-assay (Run-to-Run)

n = 5; CV = 5.0%

Summary

Features

  • This kit can measure mouse albumin with a high sensitivity (50-1000 ng/ml),
  • With a small volume of sample ( 5 μl),
  • With excellent reproducibility,
  • And rapidly (2.5hours).

Research topic

Renal disease, Animal studies

Summary

Albumin is mostly a simple hydrophilic protein present in cells and body fluids. Albumin is synthesized in the liver, and serum albumin (69 kDa, pI 4.9) occupies 56-60% of total serum proteins. Because of its large population, albumin is very important in maintaining plasma osmotic pressure. Albumin can bind hydrophobic
physiological substances e.g. fatty acids, bilirubin, and thyroxine and contributes the transfer of these substances. Concentration of albumin in serum is lowered in liver cirrhosis, malnutrition, and pyrexial diseases due to decreased biosynthesis or increased consumption of albumin in, and also by secretion into urine in renal
damage. In normal human subjects, excretion of albumin into urine is very little,about 30 mg/day, but increased in glomerulonephritis, nephritic syndrome, and diabetic nephropathy. Urinary albumin sometimes increases in pyrexia, hypertension, congestive heart failure (CHF), and urinary tract infection (UTI). Even in healthy human, a transient upraise of urinary albumin is observed after hard exercise, muscle work, bathing in high temperature water, mental excitement, stress, intake of much protein, and before menstruation. These are called physiological or functional or sports proteinuria (albuminuria). Orthostatic proteinuria (albuminuria) is observed in teenagers. Rare hereditary analbuminemia in human is really albumin deficiency.. A model analbuminemia rat has been established by Dr. Sumi Nagase which has been derived from Sprague-Dawley strain, and is called Nagase analbuminemia rat (NAR). Routine measurement of serum albumin is conveniently made with Shibayagi’s TIA assay kits for automatic analyzers. Shibayagi’s Albumin ELISA Kits can measure urinary albumin with high sensitivity, and are also applied to in vitro albumin biosynthesis system, checking of contamination with albumin in biological active substance preparations obtained by culture system, and liver transplantation experiments using NAR.

Product References (11)

References

  • Fujii M, Inoguchi T, Maeda Y, Sasaki S, Sawada F, Saito R, Kobayashi K,Sumimoto H, Takayanagi R. Pitavastatin ameliorates albuminuria and renalmesangial expansion by downregulating NOX4 in db/db mice. Kidney Int. 2007Aug;72(4):473-80. Epub 2007 Jun 13. PubMed PMID: 17568784. See more on PubMed
  • Iwashita K, Nagashima H. Rubratoxin B induces interleukin-6 secretion in mousewhite adipose tissues and 3T3-L1 adipocytes. Toxicol Lett. 2008 Nov10;182(1-3):79-83. doi: 10.1016/j.toxlet.2008.08.008. Epub 2008 Aug 29. PubMedPMID: 18804154. See more on PubMed
  • Kaburagi T, Yamano T, Fukushima Y, Yoshino H, Mito N, Sato K. Effect ofLactobacillus johnsonii La1 on immune function and serum albumin in aged andmalnourished aged mice. Nutrition. 2007 Apr;23(4):342-50. Epub 2007 Mar 23.PubMed PMID: 17367996. See more on PubMed
  • Kato H, Ishida J, Nagano K, Honjo K, Sugaya T, Takeda N, Sugiyama F, Yagami K,Fujita T, Nangaku M, Fukamizu A. Deterioration of atherosclerosis in mice lackingangiotensin II type 1A receptor in bone marrow-derived cells. Lab Invest. 2008Jul;88(7):731-9. doi: 10.1038/labinvest.2008.42. Epub 2008 May 19. PubMed PMID:18490898. See more on PubMed
  • Kato K, Kosugi T, Sato W, Arata-Kawai H, Ozaki T, Tsuboi N, Ito I, Tawada H,Yuzawa Y, Matsuo S, Kadomatsu K, Maruyama S. Growth factor Midkine is involved inthe pathogenesis of renal injury induced by protein overload containingendotoxin. Clin Exp Nephrol. 2011 Jun;15(3):346-354. doi:10.1007/s10157-011-0408-2. Epub 2011 Mar 1. PubMed PMID: 21360016. See more on PubMed
  • Mita Y, Ishihara K, Fukuchi Y, Fukuya Y, Yasumoto K. Supplementation withchromium picolinate recovers renal Cr concentration and improves carbohydratemetabolism and renal function in type 2 diabetic mice. Biol Trace Elem Res. 2005 Summer;105(1-3):229-48. PubMed PMID: 16034167. See more on PubMed
  • Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K, Kume S. Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells. 2008Jul;13(7):731-46. doi: 10.1111/j.1365-2443.2008.01201.x. Epub 2008 May 30. PubMedPMID: 18513331. See more on PubMed
  • Soto-Gutierrez A, Navarro-Alvarez N, Rivas-Carrillo JD, Tanaka K, Chen Y,Misawa H, Okitsu T, Noguchi H, Tanaka N, Kobayashi N. Construction andtransplantation of an engineered hepatic tissue using a polyaminourethane-coated nonwoven polytetrafluoroethylene fabric. Transplantation. 2007 Jan27;83(2):129-37. PubMed PMID: 17264808. See more on PubMed
  • Soto-Gutiérrez A, Navarro-Alvarez N, Zhao D, Rivas-Carrillo JD, Lebkowski J,Tanaka N, Fox IJ, Kobayashi N. Differentiation of mouse embryonic stem cells tohepatocyte-like cells by co-culture with human liver nonparenchymal cell lines.Nat Protoc. 2007;2(2):347-56. PubMed PMID: 17406596. See more on PubMed
  • Takeuchi T, Ochiya T, Takezawa T. Tissue array substratum composed ofhistological sections: a new platform for orienting differentiation of embryonic stem cells towards hepatic lineage. Tissue Eng Part A. 2008 Feb;14(2):267-74.doi: 10.1089/tea.2007.0188. PubMed PMID: 18333779. See more on PubMed
  • Tsutsui M, Ogawa S, Inada Y, Tomioka E, Kamiyoshi A, Tanaka S, Kishida T,Nishiyama M, Murakami M, Kuroda J, Hashikura Y, Miyagawa S, Satoh F, Shibata N,Tagawa Y. Characterization of cytochrome P450 expression in murine embryonic stemcell-derived hepatic tissue system. Drug Metab Dispos. 2006 Apr;34(4):696-701.Epub 2006 Jan 13. PubMed PMID: 16415121. See more on PubMed
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