United States set
Menu Shopping cart $0 Search
Distributed product

Albumin Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Rat
Cat. No. Size Price

631-04311 96 wells (1 kit) $625
PubMed Product Details
Technical Data

Cat # changed from 635-04319 to 631-04311


Sandwich ELISA, HRP-labelled antibody


Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine, Cell culture supernatant

Sample Requirements

5 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

50–1000 ng/ml

Limit of Detection

2.5 ng/ml

Intra-assay (Within-Run)

n = 5; CV = 5%

Inter-assay (Run-to-Run)

n = 5; CV = 5%


Research topic

Renal disease, Animal studies


Albumin is mostly a simple hydrophilic protein present in cells and body fluids. Albumin is synthesized in the liver, and serum albumin (69 kDa, pI 4.9) occupies 56-60% of total serum proteins. Because of its large population, albumin is very important in maintaining plasma osmotic pressure. Albumin can bind hydrophobic physiological substances e.g. fatty acids, bilirubin, and thyroxine and contributes the transfer of these substances. Concentration of albumin in serum is lowered in liver cirrhosis, malnutrition, and pyrexial diseases due to decreased biosynthesis or increased consumption of albumin in, and also by secretion into urine in renal damage. In normal human subjects, excretion of albumin into urine is very little, about 30mg/day, but increased in glomerulonephritis, nephritic syndrome, and diabetic nephropathy. Urinary albumin sometimes increases in pyrexia, hypertension, congestive heart failure (CHF), and urinary tract infection (UTI). Even in healthy human, a transient upraise of urinary albumin is observed after hard exercise, muscle work, bathing in high temperature water, mental excitement, stress, intake of much protein, and before menstruation. These are called physiological or functional or sports proteinuria (albuminuria). Orthostatic proteinuria (albuminuria) is observed in teenagers. Rare hereditary analbuminemia in human is really albumin deficiency. A model analbuminemia rat has been established by Dr. Sumi Nagase which has been derived from Sprague-Dawley strain, and is called Nagase analbuminemia rat (NAR). Routine measurement of serum albumin is conveniently made with Shibayagi’s TIA assay kits for automatic analyzers. Shibayagi’s Albumin ELISA Kits can measure urinary albumin with high sensitivity, and are also applied to in vitro albumin biosynthesis system, checking of contamination with albumin in biological active substance preparations obtained by culture system, and liver transplantation experiments using NAR.

Product References (11)


  • Odaira M, Aoki T, Miyamoto Y, Yasuhara R, Jin Z, Yu J, Nishino N, Yamada K, Kusano T, Hayashi K, Yasuda D, Koizumi T, Mitamura K, Enami Y, Niiya T, Murai N, Kato H, Shimizu Y, Kamijyo R, Kusano M. Cold preservation of the liver with oxygenation by a two-layer method. J Surg Res. 2009 Apr;152(2):209-17. doi: 10.1016/j.jss.2008.03.034. Epub 2008 Apr 23. PubMed PMID: 18701117. See more on PubMed
  • Ohtake K, Ishiyama Y, Uchida H, Muraki E, Kobayashi J. Dietary nitrite inhibits early glomerular injury in streptozotocin-induced diabetic nephropathy in rats. Nitric Oxide. 2007 Sep;17(2):75-81. doi: 10.1016/j.niox.2007.06.004. Epub 2007 Jul 4. PubMed PMID: 17681477. See more on PubMed
  • Hayashi K, Aoki T, Jin Z, Wang H, Nishino N, Kusano T, Yasuda D, Koizumi T, Enami Y, Odaira M, Yamada K, Mitamura K, Niiya T, Murai N, Kato H, Shimizu Y, Kusano M. Hepatocyte transplantation from steatotic liver in a rat model. J Surg Res. 2007 Sep;142(1):104-12. doi: 10.1016/j.jss.2006.10.048. Epub 2007 Jun 29. PubMed PMID: 17604052. See more on PubMed
  • Suzuki YA, Tomoda M, Murata Y, Inui H, Sugiura M, Nakano Y. Antidiabetic effect of long-term supplementation with Siraitia grosvenori on the spontaneously diabetic Goto-Kakizaki rat. Br J Nutr. 2007 Apr;97(4):770-5. doi: 10.1017/S0007114507381300. PubMed PMID: 17349091. See more on PubMed
  • Han SY, Kim CH, Kim HS, Jee YH, Song HK, Lee MH, Han KH, Kim HK, Kang YS, Han JY, Kim YS, Cha DR. Spironolactone prevents diabetic nephropathy through an anti-inflammatory mechanism in type 2 diabetic rats. J Am Soc Nephrol. 2006 May;17(5):1362-72. doi: 10.1681/ASN.2005111196. Epub 2006 Mar 29. PubMed PMID: 16571782. See more on PubMed
  • Han KH, Kang YS, Han SY, Jee YH, Lee MH, Han JY, Kim HK, Kim YS, Cha DR. Spironolactone ameliorates renal injury and connective tissue growth factor expression in type II diabetic rats. Kidney Int. 2006 Jul;70(1):111-20. doi: 10.1038/sj.ki.5000438. Epub 2006 May 24. PubMed PMID: 16723984. See more on PubMed
  • Yokoyama T, Ohashi K, Kuge H, Kanehiro H, Iwata H, Yamato M, Nakajima Y. In vivo engineering of metabolically active hepatic tissues in a neovascularized subcutaneous cavity. Am J Transplant. 2006 Jan;6(1):50-9. doi: 10.1111/j.1600-6143.2005.01155.x. PubMed PMID: 16433756. See more on PubMed
  • Emoto K, Tateno C, Hino H, Amano H, Imaoka Y, Asahina K, Asahara T, Yoshizato K. Efficient in vivo xenogeneic retroviral vector-mediated gene transduction into human hepatocytes. Hum Gene Ther. 2005 Oct;16(10):1168-74. doi: 10.1089/hum.2005.16.1168. PubMed PMID: 16218778. See more on PubMed
  • Ye SH, Watanabe J, Takai M, Iwasaki Y, Ishihara K. Design of functional hollow fiber membranes modified with phospholipid polymers for application in total hemopurification system. Biomaterials. 2005 Aug;26(24):5032-41. doi: 10.1016/j.biomaterials.2005.01.049. PubMed PMID: 15769539. See more on PubMed
  • Han SY, So GA, Jee YH, Han KH, Kang YS, Kim HK, Kang SW, Han DS, Han JY, Cha DR. Effect of retinoic acid in experimental diabetic nephropathy. Immunol Cell Biol. 2004 Dec;82(6):568-76. doi: 10.1111/j.1440-1711.2004.01287.x. PubMed PMID: 15550114. See more on PubMed
  • Ikegami-Kawai M, Okuda R, Nemoto T, Inada N, Takahashi T. Enhanced activity of serum and urinary hyaluronidases in streptozotocin-induced diabetic Wistar and GK rats. Glycobiology. 2004 Jan;14(1):65-72. doi: 10.1093/glycob/cwh011. Epub 2003 Oct 9. PubMed PMID: 14551218. See more on PubMed
Related Products Docs