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Albumin Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Rat
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RSHAKRAL120R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture medium, Urine

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

50–1000 ng/ml

Limit of Detection

2.5 ng/ml

Intra-assay (Within-Run)

n = 5; CV = 5%

Inter-assay (Run-to-Run)

n = 5; CV = 5%

Summary

Research topic

Animal studies, Renal disease

Summary

Albumin is mostly a simple hydrophilic protein present in cells and body fluids. Albumin is synthesized in the liver, and serum albumin (69 kDa, pI 4.9) occupies 56-60% of total serum proteins. Because of its large population, albumin is very important in maintaining plasma osmotic pressure. Albumin can bind hydrophobic physiological substances e.g. fatty acids, bilirubin, and thyroxine and contributes the transfer of these substances. Concentration of albumin in serum is lowered in liver cirrhosis, malnutrition, and pyrexial diseases due to decreased biosynthesis or increased consumption of albumin in, and also by secretion into urine in renal damage. In normal human subjects, excretion of albumin into urine is very little, about 30mg/day, but increased in glomerulonephritis, nephritic syndrome, and diabetic nephropathy. Urinary albumin sometimes increases in pyrexia, hypertension, congestive heart failure (CHF), and urinary tract infection (UTI). Even in healthy human, a transient upraise of urinary albumin is observed after hard exercise, muscle work, bathing in high temperature water, mental excitement, stress, intake of much protein, and before menstruation. These are called physiological or functional or sports proteinuria (albuminuria). Orthostatic proteinuria (albuminuria) is observed in teenagers. Rare hereditary analbuminemia in human is really albumin deficiency. A model analbuminemia rat has been established by Dr. Sumi Nagase which has been derived from Sprague-Dawley strain, and is called Nagase analbuminemia rat (NAR). Routine measurement of serum albumin is conveniently made with Shibayagi’s TIA assay kits for automatic analyzers. Shibayagi’s Albumin ELISA Kits can measure urinary albumin with high sensitivity, and are also applied to in vitro albumin biosynthesis system, checking of contamination with albumin in biological active substance preparations obtained by culture system, and liver transplantation experiments using NAR.

References to Product

References

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  • Han SY, Kim CH, Kim HS, Jee YH, Song HK, Lee MH, Han KH, Kim HK, Kang YS, Han JY, Kim YS, Cha DR. Spironolactone prevents diabetic nephropathy through an anti-inflammatory mechanism in type 2 diabetic rats. J Am Soc Nephrol. 2006 May;17 (5):1362-72
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