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Distributed product

Alpha-1-Antitrypsin in Stool Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:α-1-AT
  • Species:Human
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Cat. No. Size Price


RIC6200 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Stool

Sample Requirements

100 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

90-3,3 ng/ml

Limit of Detection

1.5 ng/ml

Intra-assay (Within-Run)

8,7%

Inter-assay (Run-to-Run)

11,9%

Spiking Recovery

80,3-105,2 %

Dilutation Linearity

76,1-92,2 %

Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!

Research topic

Immune Response, Infection and Inflammation

Summary

Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitor. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophiles. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes.

References to Product

References

  • DeBoer MD, Scharf RJ, Leite AM, Férrer A, Havt A, Pinkerton R, Lima AA, Guerrant RL. Systemic Inflammation, Growth Factors and Linear Growth in the Setting of Infection and Malnutrition. Nutrition. July 2016;
  • Exum NG, Lee GO, Olórtegui MP, et al. A Longitudinal Study of Household Water, Sanitation, and Hygiene Characteristics and Environmental Enteropathy Markers in Children Less than 24 Months in Iquitos, Peru. Am J Trop Med Hyg. 2018;98(4):995-1004
  • Fahim SM, Das S, Gazi MA, Mahfuz M, Ahmed T. Association of intestinal pathogens with faecal markers of environmental enteric dysfunction among slum-dwelling children in the first 2 years of life in Bangladesh. Trop Med Int Health. 2018;23(11):1242-1250
  • Fahim SM, Das S, Sanin KI, et al. Association of Fecal Markers of Environmental Enteric Dysfunction with Zinc and Iron Status among Children at First Two Years of Life in Bangladesh. Am J Trop Med Hyg. 2018;99(2):489-494
  • George CM, Oldja L, Biswas S, Perin J, Lee GO, Kosek M, Sack RB, Ahmed S, Haque R, Parvin T, Azmi IJ, Bhuyian SI, Talukder KA, Mohammad S, Faruque AG. Geophagy is associated with environmental enteropathy and stunting in children in rural Bangladesh. Am J Trop Med Hyg. 2015 Jun;92 (6):1117-24
  • George CM, Oldja L, Biswas S, Perin J, Sack RB, Ahmed S, Shahnaij M, Haque R, Parvin T, Azmi IJ, Bhuyian SI, Talukder KA, Faruque AG. Unsafe Child Feces Disposal is Associated with Environmental Enteropathy and Impaired Growth. J Pediatr. 2016 Jun 15;
  • Kosek M, Haque R, Lima A, Babji S, Shrestha S, Qureshi S, Amidou S, Mduma E, Lee G, Yori PP, Guerrant RL, Bhutta Z, Mason C, Kang G, Kabir M, Amour C, Bessong P, Turab A, Seidman J, Olortegui MP, Quetz J, Lang D, Gratz J, Miller M, Gottlieb M. Fecal markers of intestinal inflammation and permeability associated with the subsequent acquisition of linear growth deficits in infants. Am J Trop Med Hyg. 2013 Feb;88 (2):390-6
  • Kosek MN; MAL-ED Network Investigators.. Causal Pathways from Enteropathogens to Environmental Enteropathy: Findings from the MAL-ED Birth Cohort Study. EBioMedicine. 2017 Apr;18:109-117. doi: 10.1016/j.ebiom.2017.02.024
  • Shank JM, Kelley BR, Jackson JW, et al. The Host Antimicrobial Protein Calgranulin C Participates in the Control of Campylobacter jejuni Growth via Zinc Sequestration. Infect Immun. 2018;86(6):e00234-18. Published 2018 May 22. doi:10.1128/IAI.00234-18
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