Cat # changed from RIC6200 to IC6200
Type
Sandwich ELISA
Description
The ImmuChrom ELISA Kit is intended for the quantitative determination of alpha-1-antitrypsin stool. For in vitro diagnostic use by trained personnel in laboratories only.
Applications
Stool
Sample Requirements
100 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0-90 ng/ml
Limit of Detection
0.4 ng/ml
Intra-assay (Within-Run)
CV ≤ 11.2%
Inter-assay (Run-to-Run)
CV ≤ 14.4%
Spiking Recovery
80.3 - 105.2 %
Note
The kits are CE-IVD certified and intended for professional use.
Features
- European Union: for in vitro diagnostic use
- rest of the world: RUO
- for the determination of alpha-1- antitrypsin stool
- limit of detection 0.4 ng/ml
- internal quality control < 0.27 mg/g stool
Research topic
Immune Response, Infection and Inflammation
Summary
Alpha-1-antitrypsin is a 52 kD glycoprotein produced in the liver, intestinal macrophages, monocytes and intestinal epithelial cells. It belongs to the group of acute-phase proteins and is one of the most important proteinase inhibitors in
serum. Alpha-1-antitrypsin inhibits the proteinases trypsin and neutrophil elastase (1). A deficiency leads to increased proteolysis. Since alpha-1-antitrypsin is not appreciably broken down or resorbed in the intestine, it can be detected in the stool if the intestinal mucosa has increased permeability (“leaky gut” syndrome) (1,2).
Patients with inflammatory bowel disease show elevated levels of alpha-1-antitrypsin in their stools (4).
Taking non-steroidal anti-inflammatory drugs (e.g. aspirin, ibuprofen, diclofenac) or COX-2 inhibitors (e.g. celecoxib) can lead to enteropathies, which result in an increase in the alpha-1-antitrypsin value in the stool. Before carrying out the
determination, the corresponding medication should therefore not be taken for a period of 14 days, so as not to influence the measurement of the degree of intestinal inflammation.
In the case of diarrhea, due to the dilution effect, normal values can be measured in the stool despite the presence of inflammatory processes in the intestine.
