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Androstenedione free in saliva ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Other names:A4- androstenedione, delta4 androstenedione
  • Species:Multispecies
Cat. No. Size Price

RTC011R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA



Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

20 – 1000 pg/ml

Limit of Detection

5 pg/ml



Incubation time: 1 h at 37°C, 15 min at RT

Research topic

Autoimmunity, Reproduction, Steroid hormones, Animal studies


Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

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