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Annexin V Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Lipocortin V, Endonexin II, Calphobindin I, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant alpha, Anchorin CII, Placental protein 4, Annexin A5, Annexin-5
  • Species:Human
Cat. No. Size Price


RAF001R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.8–50 ng/ml

Limit of Detection

0.33 ng/ml

Intra-assay (Within-Run)

CV = 10.0%

Inter-assay (Run-to-Run)

CV = 11.0%

Spiking Recovery

85,00%

Dilution Linearity

89,00%

Summary

Research topic

Autoimmunity, Extracellular matrix

Summary

Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in the eukaryotic kingdom. Though structurally well investigated the in vivo function of the annexins is still unclear. They definitively belong to a family of ubiquitous cytoplasmic proteins involved in signal transduction. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site.
Thus annexin V seems to modulate the activity of some PKCs on their substrates. Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel (6). Annexin V binds to procoagulant phospholipids (Vascular anticoagulant alpha) with high affinity. Annexin V’s preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the
cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at ist cell surface. This machinery is activated during the execution of apoptosis.
Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. These findings together with the presence of Annexin V in the extracellular space depict a novel pathophysiological significance for Annexin V in vivo. Total endogenous annexin V can be detected with the present ELISA which will
offer further insights in the pathophysiological role of Annexin V.

Summary References (4)

References to Annexin V

  • Center DM, Berman JS, Kornfeld H, Theodore AC, Cruikshank WW. The lymphocyte chemoattractant factor. J Lab Clin Med. 1995 Feb;125 (2):167-72
  • Center DM, Cruikshank W. Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J Immunol. 1982 Jun;128 (6):2563-8
  • Center DM, Cruikshank WW, Berman JS, Beer DJ. Functional characteristics of histamine receptor-bearing mononuclear cells. I. Selective production of lymphocyte chemoattractant lymphokines with histamine used as a ligand. J Immunol. 1983 Oct;131 (4):1854-9
  • Cruikshank WW, Berman JS, Theodore AC, Bernardo J, Center DM. Lymphokine activation of T4+ T lymphocytes and monocytes. J Immunol. 1987 Jun 1;138 (11):3817-23
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