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Anti-OVA-IgG1 Mouse ELISA

  • Regulatory status:RUO
  • Type:Direct ELISA, Biotin-labelled antibody
  • Other names:anti-Ovalbumin
  • Species:Mouse
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RSHAKRIE040R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Direct ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.88–120 U/ml

Limit of Detection

0.094 U/ml

Intra-assay (Within-Run)

n = 5; CV = 5%

Inter-assay (Run-to-Run)

n = 3; CV = 5%

Summary

Research topic

Animal studies

Summary

IgG is the most abundant immunoglobulin and is the main antibody in the secondary immune response. IgG1, with the heavy chain 1 and molecular weight of 146kDa, occupies about 2/3 of total IgG. By limiting antigen to OVA (ovalbumin), and by measuring only IgG1, mouse immune response is simplified for elucidation. Shibayagi’s OVA-IgG1 ELISA Kit is useful for specific measurement of mouse anti-OVA IgG1 antibody.

Product References (10)

References

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  • Saito K, Torii M, Ma N, Tsuchiya T, Wang L, Hori T, Nagakubo D, Nitta N,Kanegasaki S, Hieshima K, Yoshie O, Gabazza EC, Katayama N, Shiku H, Kuribayashi K, Kato T. Differential regulatory function of resting and preactivatedallergen-specific CD4+ CD25+ regulatory T cells in Th2-type airway inflammation. J Immunol. 2008 Nov 15;181(10):6889-97. PubMed PMID: 18981108. See more on PubMed
  • Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matsuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidansuppresses the increase of plasma IgE induced by OVA-sensitization. BiochemBiophys Res Commun. 2009 Sep 25;387(3):435-9. doi: 10.1016/j.bbrc.2009.07.031.Epub 2009 Jul 14. PubMed PMID: 19607810. See more on PubMed
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