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Anti-OVA-IgG1 Mouse ELISA

  • Regulatory status:RUO
  • Type:Direct ELISA, Biotin-labelled antibody
  • Other names:anti-Ovalbumin
  • Species:Mouse
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Cat. No. Size Price


630-07669 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RSHAKRIE040R to 630-07669

Type

Direct ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.88–120 U/ml

Limit of Detection

0.094 U/ml

Intra-assay (Within-Run)

n = 5; CV = 5%

Inter-assay (Run-to-Run)

n = 3; CV = 5%

Summary

Research topic

Animal studies

Summary

IgG is the most abundant immunoglobulin and is the main antibody in the secondary immune response. IgG1, with the heavy chain 1 and molecular weight of 146kDa, occupies about 2/3 of total IgG. By limiting antigen to OVA (ovalbumin), and by measuring only IgG1, mouse immune response is simplified for elucidation. Shibayagi’s OVA-IgG1 ELISA Kit is useful for specific measurement of mouse anti-OVA IgG1 antibody.

Product References (10)

References

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  • Matsumoto K, Inoue H, Fukuyama S, Kan-O K, Eguchi-Tsuda M, Matsumoto T, Moriwaki A, Nakano T, Nakanishi Y. Frequency of Foxp3+CD4CD25+ T cells is associated with the phenotypes of allergic asthma. Respirology. 2009 Mar;14(2):187-94. doi: 10.1111/j.1440-1843.2008.01472.x. Epub 2009 Jan 23. PubMed PMID: 19192224. See more on PubMed
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  • Ichinose T, Yoshida S, Hiyoshi K, Sadakane K, Takano H, Nishikawa M, Mori I, Yanagisawa R, Kawazato H, Yasuda A, Shibamoto T. The effects of microbial materials adhered to Asian sand dust on allergic lung inflammation. Arch Environ Contam Toxicol. 2008 Oct;55(3):348-57. doi: 10.1007/s00244-007-9128-8. Epub 2008 Jan 29. PubMed PMID: 18227959. See more on PubMed
  • Ichinose T, Yoshida S, Sadakane K, Takano H, Yanagisawa R, Inoue K, Nishikawa M, Mori I, Kawazato H, Yasuda A, Shibamoto T. Effects of asian sand dust, Arizona sand dust, amorphous silica and aluminum oxide on allergic inflammation in the murine lung. Inhal Toxicol. 2008 May;20(7):685-94. doi: 10.1080/08958370801935133. PubMed PMID: 18464056. See more on PubMed
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