Aquaporin-4 Autoantibody Human ELISA

Cat. No.	Size	Price
RAQP4/96/2R	96 wells (1 kit)

Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample Requirements

50 μl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

80-1,5 u/ml

Limit of Detection

0.17 u/mL

Intra-assay (Within-Run)

7,7 %

Inter-assay (Run-to-Run)

10,5 %

References to Product

References

B. G. Weinshenker et al. Neuromyelitis optica IgG predicts relapse after longitudinally extensive transverse myelitis. Annals of Neurology 2006 59: 566 - 569
N. Isobe et al. Quantitative assays for anti-aquaporin-4 antibody with subclass analysis in neuromyelitis optica. Multiple Sclerosis Journal 2012 18: 1541 – 1551
S. Jarius et al. Testing for antibodies to human aquaporin-4 by ELISA: Sensitivity, specificity and direct comparison with immunohistochemistry. Journal of the Neurological Sciences 2012 320: 32-37
V. A. Lennon et al. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet 2004 364(9451): 2106 - 2112
V. A. Lennon et al. IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. The Journal of Experimental Medicine 2005 202: 473 - 477

References to Summary

References to Aquaporin-4 Autoantibody

Isobe N, Yonekawa T, Matsushita T, Kawano Y, Masaki K, Yoshimura S, Fichna J, Chen S, Furmaniak J, Smith BR, Kira J. Quantitative assays for anti-aquaporin-4 antibody with subclass analysis in neuromyelitis optica. Mult Scler. 2012 Nov;18 (11):1541-51
Jarius S, Franciotta D, Paul F, Bergamaschi R, Rommer PS, Ruprecht K, Ringelstein M, Aktas O, Kristoferitsch W, Wildemann B. Testing for antibodies to human aquaporin-4 by ELISA: sensitivity, specificity, and direct comparison with immunohistochemistry. J Neurol Sci. 2012 Sep 15;320 (1-2):32-7
Lennon VA, Kryzer TJ, Pittock SJ, Verkman AS, Hinson SR. IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J Exp Med. 2005 Aug 15;202 (4):473-7
Lennon VA, Wingerchuk DM, Kryzer TJ, Pittock SJ, Lucchinetti CF, Fujihara K, Nakashima I, Weinshenker BG. A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis. Lancet. 2004 Dec 11-17;364 (9451):2106-12
Weinshenker BG, Wingerchuk DM, Vukusic S, Linbo L, Pittock SJ, Lucchinetti CF, Lennon VA. Neuromyelitis optica IgG predicts relapse after longitudinally extensive transverse myelitis. Ann Neurol. 2006 Mar;59 (3):566-9

Documents

Product Datasheet (RUO)

Datasheet PDF (RUO)

Safety Information (RUO)

MSDS (RUO)