Urine, Synovial fluid, Tissue extract, Cell culture and/or animal studies
10 – 100 µl
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
All components are stable for one year, (from Invoice Date), when stored at 15–25ºC. Do not store below +4ºC. Once opened the glass vial containing sGAG standard should however be stored at +4ºC.
1 – 5 µg
Limit of Detection
- The Blyscan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).
- Test material can be assayed directly when present in a soluble form, or following papain extraction from biological materials. The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.
- The dye label used in the assay is 1, 9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.
- The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.
- Time required is 1 hour.
- Manufactured by Biocolor.
Extracellular matrix, Animal studies