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Blyscan™ sGAG Assay (Standard Kit Size)

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Other names:Glycosaminoglycan, sGAG
  • Species:Multispecies
Cat. No. Size Price

Discount B1000 110 assays (1 kit) $603,7
PubMed Product Details
Technical Data

Cat # changed from RBCB1000 to B1000


Colorimetric assay


The Blyscan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).


Cell culture and/or animal studies, Urine, Synovial fluid, Tissue extract

Sample Requirements

10 – 100 µl


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


All components are stable for one year, (from Invoice Date), when stored at 15–25ºC. Do not store below +4ºC. Once opened the glass vial containing sGAG standard should however be stored at +4ºC.

Calibration Curve

Calibration Range

1 – 5 µg

Limit of Detection

0.25 µg


Research topic

Extracellular matrix, Animal studies


The name for the assay was found using a computer based dictionary. Looking for three or more letters in sequence from ‘glycan’ we found ‘blyscan’. Blyscan is an Old English word meaning ‘to shine’ and from which the word ‘blush’, (blushing), may have been derived. This was an appropriate choice as the Blyscan Assay contains a blue dye which turns bright pink when it binds to sulphated glycosaminoglycans. The Blyscan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG). Test material can be assayed directly when present in a soluble form, or following papain extraction from biological materials. The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples. The dye label used in the assay is 1, 9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains. The assay is not suitable for small sulfated disaccharide fragments or for samples containing alginates, as these contain uronic acid.

Summary References (12)

References to Glycosaminoglycan

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  • Casu B, Lindahl U. Structure and biological interactions of heparin and heparan sulfate. Adv Carbohydr Chem Biochem. 2001;57:159-206. doi: 10.1016/s0065-2318(01)57017-1. PMID: 11836942. See more on PubMed
  • Couchman JR, Pataki CA. An introduction to proteoglycans and their localization. J Histochem Cytochem. 2012 Dec;60(12):885-97. doi: 10.1369/0022155412464638. Epub 2012 Sep 26. PMID: 23019015; PMCID: PMC3527888. See more on PubMed
  • Gandhi NS, Mancera RL. The structure of glycosaminoglycans and their interactions with proteins. Chem Biol Drug Des. 2008 Dec;72(6):455-82. doi: 10.1111/j.1747-0285.2008.00741.x. PMID: 19090915. See more on PubMed
  • Holt CE, Dickson BJ. Sugar codes for axons? Neuron. 2005 Apr 21;46(2):169-72. doi: 10.1016/j.neuron.2005.03.021. PMID: 15848796; PMCID: PMC3687205. See more on PubMed
  • Iozzo RV, San Antonio JD. Heparan sulfate proteoglycans: heavy hitters in the angiogenesis arena. J Clin Invest. 2001 Aug;108(3):349-55. doi: 10.1172/JCI13738. PMID: 11489925; PMCID: PMC209371. See more on PubMed
  • Jackson RL, Busch SJ, Cardin AD. Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes. Physiol Rev. 1991 Apr;71(2):481-539. doi: 10.1152/physrev.1991.71.2.481. PMID: 2006221. See more on PubMed
  • Liu D, Shriver Z, Qi Y, Venkataraman G, Sasisekharan R. Dynamic regulation of tumor growth and metastasis by heparan sulfate glycosaminoglycans. Semin Thromb Hemost. 2002 Feb;28(1):67-78. doi: 10.1055/s-2002-20565. PMID: 11885027. See more on PubMed
  • Sanderson RD. Heparan sulfate proteoglycans in invasion and metastasis. Semin Cell Dev Biol. 2001 Apr;12(2):89-98. doi: 10.1006/scdb.2000.0241. PMID: 11292374. See more on PubMed
  • Sasisekharan R, Shriver Z, Venkataraman G, Narayanasami U. Roles of heparan- sulphate glycosaminoglycans in cancer. Nat Rev Cancer. 2002 Jul;2(7):521-8. doi: 10.1038/nrc842. PMID: 12094238. See more on PubMed
  • Tímár J, Lapis K, Dudás J, Sebestyén A, Kopper L, Kovalszky I. Proteoglycans and tumor progression: Janus-faced molecules with contradictory functions in cancer. Semin Cancer Biol. 2002 Jun;12(3):173-86. doi: 10.1016/S1044-579X(02)00021-4. PMID: 12083848. See more on PubMed
  • Yip GW, Smollich M, Götte M. Therapeutic value of glycosaminoglycans in cancer. Mol Cancer Ther. 2006 Sep;5(9):2139-48. doi: 10.1158/1535-7163.MCT-06-0082. PMID: 16985046. See more on PubMed
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