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C-Peptide (U-type) Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Species:Mouse
Cat. No. Size Price

635-07239/631-07231 96 wells (1 kit) $665
PubMed Product Details
Technical Data

Cat # changed from 635-07239 to 631-07231


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Sample Requirements

10 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

30–3 000 pg/ml

Limit of Detection

1.5 pg/ml


Research topic

Diabetology - Insulin, C-Peptide, Proinsulin, Animal studies


Insulin is first synthesized as a single chain polypeptide, proinsulin, then three disulfide bonds are formed, and finally divided into insulin and C-peptide through enzymatic splitting. Mouse C-peptide 1 is a single chain peptide composed of 29 amino acids, while C-peptide 2 is composed of 31 residues. C-peptide is secreted together with insulin. The role of C-peptide has been considered to keep the best configuration to form three disulfide bonds, and has no biological activity, however, recent studies revealed that C-peptide can bind, probably, a G-proteincoupling specific receptor present on the surface of endothelial cells, kidney microtubule cells and fibroblasts, resulting in activation of calcium-dependent intracellular signaling, activation of Na+-K+-ATPase, and enhancement of NO synthesis. Administration of C-peptide to DM1 patients enhances blood circulation in the skeletal muscle and skin, and also minimizes kidney glomerular hyperfiltration, decreasing albumin excretion into urine, and also improves nervous function, indicating that C-peptide should be given together with insulin to DM1 patients. Important region to bind receptor has been reported to be C-terminal pentapeptide (27-31).
The biological half life of C-peptide is several times longer than that of insulin. Measurement of C-peptide is useful in estimation of pancreatic function for insulin synthesis and secretion. Urinary C-peptide concentration is well correlated to its blood level. C-peptide measurement is also useful in estimation of insulin secretion by cultured islet of Langerhans because very often insulin is added to the culture medium, and it is difficult to discriminate secreted insulin from added insulin. As Shibayagi’s kit recognizes the common sequences between C-peptide 1 and 2, it can measure total amount of C-peptide.

Product References (6)


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  • Liu MJ, Shin S, Li N, Shigihara T, Lee YS, Yoon JW, Jun HS. Prolonged remission of diabetes by regeneration of beta cells in diabetic mice treated with recombinant adenoviral vector expressing glucagon-like peptide-1. Mol Ther. 2007 Jan;15(1):86-93. doi: 10.1038/sj.mt.6300005. PubMed PMID: 17164779. See more on PubMed
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  • Torisu T, Sato N, Yoshiga D, Kobayashi T, Yoshioka T, Mori H, Iida M, Yoshimura A. The dual function of hepatic SOCS3 in insulin resistance in vivo. Genes Cells. 2007 Feb;12(2):143-54. doi: 10.1111/j.1365-2443.2007.01044.x. PubMed PMID: 17295835. See more on PubMed
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Summary References (10)

References to C-Peptide

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  • Beischer W, Keller L, Maas M, Scherfer E, Pfeiffer EF. Human C-peptide. Part I: Radioimmunoassay. Klin Wochenschr. 1976 Aug 1;54 (15):709-15
  • Blix PM, Boddie-Willis C, Landau RL, Rochman H, Rubenstein AH. Urinary C-peptide: an indicator of beta-cell secretion under different metabolic conditions. J Clin Endocrinol Metab. 1982 Mar;54 (3):574-80
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  • Horwitz DL, Starr JI, Mako ME, Blackard WG, Rubenstein AH. Proinsulin, insulin, and C-peptide concentrations in human portal and peripheral blood. J Clin Invest. 1975 Jun;55 (6):1278-83
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  • Rendell M. C-peptide levels as a criterion in treatment of maturity-onset diabetes. J Clin Endocrinol Metab. 1983 Dec;57 (6):1198-206
  • Rubenstein AH, Steiner DF, Horwitz DL, Mako ME, Block MB, Starr JI, Kuzuya H, Melani F. Clinical significance of circulating proinsulin and C-peptide. Recent Prog Horm Res. 1976;33:435-75
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