United States set
Menu Shopping cart $0 Search
Distributed product

Calprotectin ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:MRP8/14, Myeloid Related Protein 8/14, Calprotectin
  • Species:Human
Cat. No. Size Price

Discount IC7300 96 wells (1 kit) $640
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody




Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Limit of Detection

1.0 ng/ml

Intra-assay (Within-Run)

n = 10; CV = 5.0 %

Inter-assay (Run-to-Run)

n = 10; CV = 7.3 %

Spiking Recovery

94.69 %

Dilution Linearity

106.77 %



  • European Union: for in vitro diagnostic use
  • rest of the world: for research use only!
  • The kit is intended for the quantitative determination of Calprotectin in stool.
  • Assay format is 96 wells
  • Components of the kit are provided ready to use or concentrated

Research topic

Autoimmunity, Diabetology - Other Relevant Products, Inflammatory bowel disease


Human calprotectin is a dimer which consists of the sub units S100A8 (10.835 kDa) and S100A9 (13.242 kDa). The monomers are able to bind calcium. The complex is located in the cytosol of neutrophils and is excreted to the intestine during inflammation. The concentration in faecal samples correlates with the severity of inflammatory processes in the intestine. The complex is resistant against enzymatic degradation. The measurement of Calprotectin represents an easy non-invasive analysis of intestinal inflammation with the possibility to differentiate between noninflammatory irritable bowel disease and intestinal inflammation accompanied with morphological alteration of the intestinal mucosa.
In case of viral or bacterial infections of the gut the concentration of calprotectin in faeces is increased. Therefore, before further diagnostic clarification of a chronic inflammatory bowel disease and in case of an appropriate / corresponding clinic, the exclusion of an infectious cause (e.g. detection of pathogens, detection of pathogen-specific antibodies) is always necessary.
The use of non-steroidal anti-inflammatory drugs (e.g. aspirin, ibuprofen, diclofenac) or COX-2 inhibitors (e.g. celecoxib) can lead to enteropathies that lead to an increase in the calprotectin level in the stool. Before carrying out the determination, the corresponding medication should therefore not be taken for a period of 14 days if possible so as not to influence the measurement of the degree of intestinal inflammation. Administration of pantoprazole (20 mg/day) can lead to increased calprotectin levels. A measurement of calprotectin should be carried out 3 weeks after discontinuation of the medication.


  • inflammatory processes in the intestine
  • clinical course of M. Crohn, colitis ulcerosa or after polyps removal
  • safe differentiation between organic disorder of the intestine (chronic inflammatory bowel disease, infections, polyps, colon cancer) and functional disorder of the intestine (irritable bowel syndrome)

The ImmuChrom complete calprotectin kit allows an easy, rapid and precise quantitative determination of calprotectin in biological samples. The kit includes all reagents ready to use for preparation of the samples.

Summary References (16)

References to S100A8/A9

  • Altwegg LA, Neidhart M, Hersberger M, Muller S, Eberli FR, Corti R, Roffi M, Sutsch G, Gay S, von Eckardstein A, Wischnewsky MB, Luscher TF, Maier W. Myeloid-related protein 8/14 complex is released by monocytes and granulocytes at the site of coronary occlusion: a novel, early, and sensitive marker of acute coronary syndromes. Eur Heart J. 2007 Apr;28 (8):941-8
  • Andres Cerezo L, Mann H, Pecha O, Plestilova L, Pavelka K, Vencovsky J, Senolt L. Decreases in serum levels of S100A8/9 (calprotectin) correlate with improvements in total swollen joint count in patients with recent-onset rheumatoid arthritis. Arthritis Res Ther. 2011;13 (4):R122
  • Andres Cerezo L, Mann H, Pecha O, Plestilova L, Pavelka K, Vencovsky J, Senolt L. Decreases in serum levels of S100A8/9 (calprotectin) correlate with improvements in total swollen joint count in patients with recent-onset rheumatoid arthritis. Arthritis Res Ther. 2011;13 (4):R122
  • Bealer JF, Colgin M. S100A8/A9: a potential new diagnostic aid for acute appendicitis. Acad Emerg Med. 2010 Mar;17 (3):333-6
  • Bhardwaj RS, Zotz C, Zwadlo-Klarwasser G, Roth J, Goebeler M, Mahnke K, Falk M, Meinardus-Hager G, Sorg C. The calcium-binding proteins MRP8 and MRP14 form a membrane-associated heterodimer in a subset of monocytes/macrophages present in acute but absent in chronic inflammatory lesions. Eur J Immunol. 1992 Jul;22 (7):1891-7
  • Chen YS, Yan W, Geczy CL, Brown MA, Thomas R. Serum levels of soluble receptor for advanced glycation end products and of S100 proteins are associated with inflammatory, autoantibody, and classical risk markers of joint and vascular damage in rheumatoid arthritis. Arthritis Res Ther. 2009;11 (2):R39
  • Ghavami S, Rashedi I, Dattilo BM, Eshraghi M, Chazin WJ, Hashemi M, Wesselborg S, Kerkhoff C, Los M. S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway. J Leukoc Biol. 2008 Jun;83 (6):1484-92
  • Heller F, Frischmann S, Grunbaum M, Zidek W, Westhoff TH. Urinary calprotectin and the distinction between prerenal and intrinsic acute kidney injury. Clin J Am Soc Nephrol. 2011 Oct;6 (10):2347-55
  • Limburg PJ, Ahlquist DA, Sandborn WJ, Mahoney DW, Devens ME, Harrington JJ, Zinsmeister AR. Fecal calprotectin levels predict colorectal inflammation among patients with chronic diarrhea referred for colonoscopy. Am J Gastroenterol. 2000 Oct;95 (10):2831-7
  • Lood C, Stenstrom M, Tyden H, Gullstrand B, Kallberg E, Leanderson T, Truedsson L, Sturfelt G, Ivars F, Bengtsson AA. Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. Arthritis Res Ther. 2011;13 (2):R60
  • Mortensen OH, Nielsen AR, Erikstrup C, Plomgaard P, Fischer CP, Krogh-Madsen R, Lindegaard B, Petersen AM, Taudorf S, Pedersen BK. Calprotectin--a novel marker of obesity. PLoS One. 2009;4 (10):e7419
  • Striz I, Trebichavsky I. Calprotectin - a pleiotropic molecule in acute and chronic inflammation. Physiol Res. 2004;53 (3):245-53
  • Terrin G, Passariello A, Manguso F, Salvia G, Rapacciuolo L, Messina F, Raimondi F, Canani RB. Serum calprotectin: an antimicrobial peptide as a new marker for the diagnosis of sepsis in very low birth weight newborns. Clin Dev Immunol. 2011;2011:291085
  • Tibble JA, Sigthorsson G, Foster R, Forgacs I, Bjarnason I. Use of surrogate markers of inflammation and Rome criteria to distinguish organic from nonorganic intestinal disease. Gastroenterology. 2002 Aug;123 (2):450-60
  • Vogl T, Roth J, Sorg C, Hillenkamp F, Strupat K. Calcium-induced noncovalently linked tetramers of MRP8 and MRP14 detected by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry. J Am Soc Mass Spectrom. 1999 Nov;10 (11):1124-30
  • Yong HY, Moon A. Roles of calcium-binding proteins, S100A8 and S100A9, in invasive phenotype of human gastric cancer cells. Arch Pharm Res. 2007 Jan;30 (1):75-81
Related Products Docs