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Caspase-9 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, 2nd HRPlabelled antibody
  • Other names:ICE like apoptotic protease 6, Apoptotic protease Mch-6, Apoptotic protease activating factor 3
  • Species:Human
Cat. No. Size Price

RAF007R 96 wells (1 kit) $656,21
PubMed Product Details
Technical Data


Sandwich ELISA, 2nd HRPlabelled antibody


Serum, Cell culture supernatant, Cell culture lysate

Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.6–100 ng/ml

Limit of Detection

0.4 ng/ml

Intra-assay (Within-Run)

CV = 6.6%

Inter-assay (Run-to-Run)

CV = 9.0%

Spiking Recovery


Dilution Linearity



Research topic

Apoptosis, Immune Response, Infection and Inflammation


Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.
Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP, which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP.
Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation.
Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and –7 are targets of caspase-9 activity . Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a socalled apoptosome, amplifying the caspase cascade. Feedback cleavage of caspase-9 by caspase-3 again significantly increases the activity of the apoptosome .
Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimer´s disease, Huntington´s disease and osteoarthritis .

Product References (1)


  • Gornowicz A, Bielawska A, Szymanowski W, Gabryel-Porowska H, Czarnomysy R, Bielawski K. Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells. Oncol Lett. 2018 Feb;15(2):2340-2348. doi: 10.3892/ol.2017.7623. Epub 2017 Dec 14. PubMed PMID: 29434943. PubMed CentralPMCID: PMC5776928. See more on PubMed
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