Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

cel-miR-39-3p miREIA

  • Regulatory status:RUO
  • Type:miREIA – miRNA enzyme immunoassay
  • Species:C. Elegans
Please select your region to see available products and prices.
Cat. No. Size Price


New RDM0000C 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Exogenous spike-in control

Sample Requirements

10 µl/well

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

12.5 – 0.39 amol/μl

Limit of Detection

0.13 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 8.3%

Inter-assay (Run-to-Run)

n = 5,
CV = 7.4%

Spiking Recovery

108.0%

Dilutation Linearity

95.1%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 2.5 hours
  • The kit measures concentration of exogenous cel-miR-39-3p spiked in RNA isolates
  • Assay format is 96 wells
  • Standard is synthetic miRNA-based
  • Components of the kit are provided ready to use, concentrated or dried

Research topic

Exogenous control, Spike-in control

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

Concentration of target miRNA measured by miREIA can be affected by efficiency of RNA isolation. Therefore, it is recommended to normalize the measured concentration of target miRNA to the efficiency of RNA isolation using an exogenous control. Efficiency of RNA isolation can be monitored by adding a defined amount of synthetic non-human miRNA, e.g. cel-miR-39-3p miRNA, to the sample during RNA isolation. The synthetic RNA is processed the same way as the target RNA present in the samples. After RNA isolation, concentration of exogenous cel-miR-39-3p added to the samples is measured by cel-miR-39-3p miREIA in parallel with the concentration of the target miRNA. To calculate the coefficient of isolation efficiency, the amount of cel-miR-39-3p added to the samples prior to isolation is divided by the concentration of cel-miR-39-3p measured by miREIA. Finally, the concentration of target miRNA measured by miREIA is multiplied by the coefficient of isolation efficiency for every sample.

If such a normalization is applied, then, each RNA isolate sample is measured by two miREIA kits: one kit for determination of the target miRNA (samples are diluted as recommended in the corresponding datasheet) and by a second kit, cel-miR-39-3p miREIA (samples are diluted 10-fold as written in page 15).

Related Products Documents