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Chemerin Human ELISA

Chemerin Human ELISA
  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Tezarotene induced gene 2, TIG2, Retinoic acid receptor responder 2, RERRES2
  • Species:Human
United States orders are shipped from our US branch, BioVendor, LLC
Cat. No. Size Price


RD191136200R 96 wells (1 kit) $567
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma

Sample Requirements

5 μl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.25–8 ng/ml

Limit of Detection

0.1 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.0%

Inter-assay (Run-to-Run)

n = 6; CV = 7.6%

Spiking Recovery

96,40%

Dilution Linearity

103,20%

Crossreactivity

  • bovine Non-detectable
  • cat Not tested
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • rabbit None-specific binding
Summary

Features

  • It is intended for research use only.
  • The total assay time is less than 3.5 hours.
  • The kit measures chemerin in serum and plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Standard is recombinant protein based
  • Quality Controls are human serum based. No animal sera are used.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Energy metabolism and body weight regulation

Product References (51)

References

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Summary References (7)

References to Chemerin

  • Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, Walder K, Segal D. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. 2007 Oct;148 (10):4687-94
  • Goralski KB, McCarthy TC, Hanniman EA, Zabel BA, Butcher EC, Parlee SD, Muruganandan S, Sinal CJ. Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism. J Biol Chem. 2007 Sep 21;282 (38):28175-88
  • MacDougald OA, Burant CF. The rapidly expanding family of adipokines. Cell Metab. 2007 Sep;6 (3):159-61
  • Parolini S, Santoro A, Marcenaro E, Luini W, Massardi L, Facchetti F, Communi D, Parmentier M, Majorana A, Sironi M, Tabellini G, Moretta A, Sozzani S. The role of chemerin in the colocalization of NK and dendritic cell subsets into inflamed tissues. Blood. 2007 May 1;109 (9):3625-32
  • Roh SG, Song SH, Choi KC, Katoh K, Wittamer V, Parmentier M, Sasaki S. Chemerin--a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun. 2007 Nov 3;362 (4):1013-8
  • Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Kitazawa R, Iida K, Okimura Y, Kaji H, Kitazawa S, Kasuga M, Chihara K. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett. 2008 Mar 5;582 (5):573-8
  • Wittamer V, Franssen JD, Vulcano M, Mirjolet JF, Le Poul E, Migeotte I, Brezillon S, Tyldesley R, Blanpain C, Detheux M, Mantovani A, Sozzani S, Vassart G, Parmentier M, Communi D. Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Med. 2003 Oct 6;198 (7):977-85
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