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Chemerin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Tezarotene induced gene 2, TIG2, Retinoic acid receptor responder 2, RERRES2
  • Species:Human
Cat. No. Size Price


RD191136200R 96 wells (1 kit) $531
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma

Sample Requirements

5 μl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.25–8 ng/ml

Limit of Detection

0.1 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.0%

Inter-assay (Run-to-Run)

n = 6; CV = 7.6%

Spiking Recovery

96,40%

Dilution Linearity

103,20%

Crossreactivity

  • bovine Non-detectable
  • cat Not tested
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • rabbit None-specific binding
Summary

Features

  • It is intended for research use only.
  • The total assay time is less than 3.5 hours.
  • The kit measures chemerin in serum and plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Standard is recombinant protein based
  • Quality Controls are human serum based. No animal sera are used.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Energy metabolism and body weight regulation

Product References (51)

References

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  • Sawicka K, Michalska-Jakubus M, Potembska E, Kowal M, Pietrzak A, Krasowska D. Visfatin and chemerin levels correspond with inflammation and might reflect the bridge between metabolism, inflammation and fibrosis in patients with systemic sclerosis. Postepy Dermatol Alergol. 2019 Oct;36(5):551-565. doi: 10.5114/ada.2018.79104. Epub 2019 Nov 6. PubMed PMID: 31839772. PubMed CentralPMCID: PMC6906965. See more on PubMed
  • Horn P, von Loeffelholz C, Forkert F, Stengel S, Reuken P, Aschenbach R, Stallmach A, Bruns T. Low circulating chemerin levels correlate with hepatic dysfunction and increased mortality in decompensated liver cirrhosis. Sci Rep. 2018 Jun 18;8(1):9242. doi: 10.1038/s41598-018-27543-6. PubMed PMID: 29915268. PubMed CentralPMCID: PMC6006249. See more on PubMed
  • Menzel J, Biemann R, Aleksandrova K, Schulze MB, Boeing H, Isermann B, Weikert C. The cross-sectional association between chemerin and bone health in peri/pre and postmenopausal women: results from the EPIC-Potsdam study. Menopause. 2018 May;25(5):574-578. doi: 10.1097/GME.0000000000001041. PubMed PMID: 29206770. PubMed CentralPMCID: PMC5908253. See more on PubMed
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  • Ghallab NA, Shaker OG. Serum and salivary levels of chemerin and MMP-9 in oral squamous cell carcinoma and oral premalignant lesions. Clin Oral Investig. 2017 Apr;21(3):937-947. doi: 10.1007/s00784-016-1846-8. Epub 2016 May 10. PubMed PMID: 27161218. See more on PubMed
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  • Eichelmann F, Weikert C, di Giuseppe R, Biemann R, Isermann B, Schulze MB, Boeing H, Aleksandrova K. Methodological utility of chemerin as a novel biomarker of immunity and metabolism. Endocr Connect. 2017 Jul;6(5):340-347. doi: 10.1530/EC-17-0098. Epub 2017 Jun 7. PubMed PMID: 28592510. PubMed CentralPMCID: PMC5510453. See more on PubMed
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  • Görkem Ü, Küçükler FK, Toğrul C, Güngör T. Are adipokines associated with gestational diabetes mellitus?. J Turk Ger Gynecol Assoc. 2016 Dec 1;17(4):186-190. doi: 10.5152/jtgga.2016.16112. eCollection 2016. PubMed PMID: 27990086. PubMed CentralPMCID: PMC5147756. See more on PubMed
  • Engin-Üstün Y, Çağlayan EK, Göçmen AY, Polat MF. Postmenopausal Osteoporosis Is Associated with Serum Chemerin and Irisin but Not with Apolipoprotein M Levels. J Menopausal Med. 2016 Aug;22(2):76-9. doi: 10.6118/jmm.2016.22.2.76. Epub 2016 Aug 30. PubMed PMID: 27617241. PubMed CentralPMCID: PMC5016507. See more on PubMed
  • Flehmig G, Scholz M, Klöting N, Fasshauer M, Tönjes A, Stumvoll M, Youn BS, Blüher M. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation. PLoS One. 2014 Jun 26;9(6):e99785. doi: 10.1371/journal.pone.0099785. eCollection 2014. PubMed PMID: 24968098. PubMed CentralPMCID: PMC4072672. See more on PubMed
  • Coimbra S, Brandão Proença J, Santos-Silva A, Neuparth MJ. Adiponectin, leptin, and chemerin in elderly patients with type 2 diabetes mellitus: a close linkage with obesity and length of the disease. Biomed Res Int. 2014;2014:701915. doi: 10.1155/2014/701915. Epub 2014 Jul 2. PubMed PMID: 25105135. PubMed CentralPMCID: PMC4101968. See more on PubMed
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  • Imai K, Takai K, Hanai T, Shiraki M, Suzuki Y, Hayashi H, Naiki T, Nishigaki Y, Tomita E, Shimizu M, Moriwaki H. Impact of serum chemerin levels on liver functional reserves and platelet counts in patients with hepatocellular carcinoma. Int J Mol Sci. 2014 Jun 25;15(7):11294-306. doi: 10.3390/ijms150711294. PubMed PMID: 24968270. PubMed CentralPMCID: PMC4139783. See more on PubMed
  • Meric M, Soylu K, Avci B, Yuksel S, Gulel O, Yenercag M, Coksevim M, Uzun A. Evaluation of plasma chemerin levels in patients with non-dipper blood pressure patterns. Med Sci Monit. 2014 Apr 28;20:698-705. doi: 10.12659/MSM.890784. PubMed PMID: 24769499. PubMed CentralPMCID: PMC4010600. See more on PubMed
  • Aronis KN, Sahin-Efe A, Chamberland JP, Spiro A 3rd, Vokonas P, Mantzoros CS. Chemerin levels as predictor of acute coronary events: a case-control study nested within the veterans affairs normative aging study. Metabolism. 2014 Jun;63(6):760-6. doi: 10.1016/j.metabol.2014.02.013. Epub 2014 Feb 25. PubMed PMID: 24684821. See more on PubMed
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  • Polyzos SA, Kountouras J, Anastasilakis AD, Geladari EV, Mantzoros CS. Irisin in patients with nonalcoholic fatty liver disease. Metabolism. 2014 Feb;63(2):207-17. doi: 10.1016/j.metabol.2013.09.013. Epub 2013 Oct 18. PubMed PMID: 24140091. See more on PubMed
  • Luque-Ramírez M, Martínez-García MÁ, Montes-Nieto R, Fernández-Durán E, Insenser M, Alpañés M, Escobar-Morreale HF. Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge. Hum Reprod. 2013 Jul;28(7):1908-18. doi: 10.1093/humrep/det097. Epub 2013 Apr 4. PubMed PMID: 23559188. See more on PubMed
  • Chamberland JP, Berman RL, Aronis KN, Mantzoros CS. Chemerin is expressed mainly in pancreas and liver, is regulated by energy deprivation, and lacks day/night variation in humans. Eur J Endocrinol. 2013 Sep 13;169(4):453-62. doi: 10.1530/EJE-13-0098. Print 2013 Oct. PubMed PMID: 23904282. PubMed CentralPMCID: PMC3798003. See more on PubMed
  • Neuparth MJ, Proença JB, Santos-Silva A, Coimbra S. Adipokines, oxidized low-density lipoprotein, and C-reactive protein levels in lean, overweight, and obese portuguese patients with type 2 diabetes. ISRN Obes. 2013 Jan 17;2013:142097. doi: 10.1155/2013/142097. eCollection 2013. PubMed PMID: 24634792. PubMed CentralPMCID: PMC3901968. See more on PubMed
  • Ouwens DM, Bekaert M, Lapauw B, Van Nieuwenhove Y, Lehr S, Hartwig S, Calders P, Kaufman JM, Sell H, Eckel J, Ruige JB. Chemerin as biomarker for insulin sensitivity in males without typical characteristics of metabolic syndrome. Arch Physiol Biochem. 2012 Jul;118(3):135-8. doi: 10.3109/13813455.2012.654800. Epub 2012 Feb 15. PubMed PMID: 22335466. See more on PubMed
  • Reverchon M, Cornuau M, Ramé C, Guerif F, Royère D, Dupont J. Chemerin inhibits IGF-1-induced progesterone and estradiol secretion in human granulosa cells. Hum Reprod. 2012 Jun;27(6):1790-800. doi: 10.1093/humrep/des089. Epub 2012 Mar 23. PubMed PMID: 22447628. See more on PubMed
  • Chakaroun R, Raschpichler M, Klöting N, Oberbach A, Flehmig G, Kern M, Schön MR, Shang E, Lohmann T, Dreßler M, Fasshauer M, Stumvoll M, Blüher M. Effects of weight loss and exercise on chemerin serum concentrations and adipose tissue expression in human obesity. Metabolism. 2012 May;61(5):706-14. doi: 10.1016/j.metabol.2011.10.008. Epub 2011 Dec 2. PubMed PMID: 22136911. See more on PubMed
  • Eisinger K, Bauer S, Schäffler A, Walter R, Neumann E, Buechler C, Müller-Ladner U, Frommer KW. Chemerin induces CCL2 and TLR4 in synovial fibroblasts of patients with rheumatoid arthritis and osteoarthritis. Exp Mol Pathol. 2012 Feb;92(1):90-6. doi: 10.1016/j.yexmp.2011.10.006. Epub 2011 Oct 19. PubMed PMID: 22037282. See more on PubMed
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  • Stepan H, Philipp A, Roth I, Kralisch S, Jank A, Schaarschmidt W, Lössner U, Kratzsch J, Blüher M, Stumvoll M, Fasshauer M. Serum levels of the adipokine chemerin are increased in preeclampsia during and 6 months after pregnancy. Regul Pept. 2011 Jun 7;168(1-3):69-72. doi: 10.1016/j.regpep.2011.03.005. Epub 2011 Apr 12. PubMed PMID: 21477622. See more on PubMed
  • El-Mesallamy HO, El-Derany MO, Hamdy NM. Serum omentin-1 and chemerin levels are interrelated in patients with Type 2 diabetes mellitus with or without ischaemic heart disease. Diabet Med. 2011 Oct;28(10):1194-200. doi: 10.1111/j.1464-5491.2011.03353.x. PubMed PMID: 21668495. See more on PubMed
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  • Yilmaz Y, Yonal O, Kurt R, Alahdab YO, Eren F, Ozdogan O, Celikel CA, Imeryuz N, Kalayci C, Avsar E. Serum levels of omentin, chemerin and adipsin in patients with biopsy-proven nonalcoholic fatty liver disease. Scand J Gastroenterol. 2011 Jan;46(1):91-7. doi: 10.3109/00365521.2010.516452. Epub 2010 Sep 1. PubMed PMID: 20809771. See more on PubMed
  • Tönjes A, Fasshauer M, Kratzsch J, Stumvoll M, Blüher M. Adipokine pattern in subjects with impaired fasting glucose and impaired glucose tolerance in comparison to normal glucose tolerance and diabetes. PLoS One. 2010 Nov 9;5(11):e13911. doi: 10.1371/journal.pone.0013911. PubMed PMID: 21085476. PubMed CentralPMCID: PMC2976690. See more on PubMed
  • Pfau D, Stepan H, Kratzsch J, Verlohren M, Verlohren HJ, Drynda K, Lössner U, Blüher M, Stumvoll M, Fasshauer M. Circulating levels of the adipokine chemerin in gestational diabetes mellitus. Horm Res Paediatr. 2010;74(1):56-61. doi: 10.1159/000282114. Epub 2010 Apr 29. PubMed PMID: 20424419. See more on PubMed
  • Klöting N, Fasshauer M, Dietrich A, Kovacs P, Schön MR, Kern M, Stumvoll M, Blüher M. Insulin-sensitive obesity. Am J Physiol Endocrinol Metab. 2010 Sep;299(3):E506-15. doi: 10.1152/ajpendo.00586.2009. Epub 2010 Jun 22. PubMed PMID: 20570822. See more on PubMed
  • Saremi A, Shavandi N, Parastesh M, Daneshmand H. Twelve-week aerobic training decreases chemerin level and improves cardiometabolic risk factors in overweight and obese men. Asian J Sports Med. 2010 Sep;1(3):151-8. doi: 10.5812/asjsm.34860. PubMed PMID: 22375203. PubMed CentralPMCID: PMC3289180. See more on PubMed
  • Sell H, Divoux A, Poitou C, Basdevant A, Bouillot JL, Bedossa P, Tordjman J, Eckel J, Clément K. Chemerin correlates with markers for fatty liver in morbidly obese patients and strongly decreases after weight loss induced by bariatric surgery. J Clin Endocrinol Metab. 2010 Jun;95(6):2892-6. doi: 10.1210/jc.2009-2374. Epub 2010 Apr 7. PubMed PMID: 20375212. See more on PubMed
  • Ress C, Tschoner A, Engl J, Klaus A, Tilg H, Ebenbichler CF, Patsch JR, Kaser S. Effect of bariatric surgery on circulating chemerin levels. Eur J Clin Invest. 2010 Mar;40(3):277-80. doi: 10.1111/j.1365-2362.2010.02255.x. Epub 2010 Jan 25. PubMed PMID: 20105226. See more on PubMed
  • Pfau D, Bachmann A, Lössner U, Kratzsch J, Blüher M, Stumvoll M, Fasshauer M. Serum levels of the adipokine chemerin in relation to renal function. Diabetes Care. 2010 Jan;33(1):171-3. doi: 10.2337/dc09-1351. Epub 2009 Oct 16. PubMed PMID: 19837789. PubMed CentralPMCID: PMC2797967. See more on PubMed
  • Sell H, Laurencikiene J, Taube A, Eckardt K, Cramer A, Horrighs A, Arner P, Eckel J. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes. 2009 Dec;58(12):2731-40. doi: 10.2337/db09-0277. Epub 2009 Aug 31. PubMed PMID: 19720798. PubMed CentralPMCID: PMC2780878. See more on PubMed
  • Stejskal D, Karpisek M, Hanulova Z, Svestak M. Chemerin is an independent marker of the metabolic syndrome in a Caucasian population--a pilot study. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2008 Dec;152(2):217-21. doi: 10.5507/bp.2008.033. PubMed PMID: 19219210. See more on PubMed
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Summary References (7)

References to Chemerin

  • Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, Walder K, Segal D. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. 2007 Oct;148 (10):4687-94
  • Goralski KB, McCarthy TC, Hanniman EA, Zabel BA, Butcher EC, Parlee SD, Muruganandan S, Sinal CJ. Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism. J Biol Chem. 2007 Sep 21;282 (38):28175-88
  • MacDougald OA, Burant CF. The rapidly expanding family of adipokines. Cell Metab. 2007 Sep;6 (3):159-61
  • Parolini S, Santoro A, Marcenaro E, Luini W, Massardi L, Facchetti F, Communi D, Parmentier M, Majorana A, Sironi M, Tabellini G, Moretta A, Sozzani S. The role of chemerin in the colocalization of NK and dendritic cell subsets into inflamed tissues. Blood. 2007 May 1;109 (9):3625-32
  • Roh SG, Song SH, Choi KC, Katoh K, Wittamer V, Parmentier M, Sasaki S. Chemerin--a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun. 2007 Nov 3;362 (4):1013-8
  • Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Kitazawa R, Iida K, Okimura Y, Kaji H, Kitazawa S, Kasuga M, Chihara K. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett. 2008 Mar 5;582 (5):573-8
  • Wittamer V, Franssen JD, Vulcano M, Mirjolet JF, Le Poul E, Migeotte I, Brezillon S, Tyldesley R, Blanpain C, Detheux M, Mantovani A, Sozzani S, Vassart G, Parmentier M, Communi D. Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Med. 2003 Oct 6;198 (7):977-85
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