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Chromogranin A Human ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Other names:Pituitary secretory protein I , CgA, SP-I, CHGA
  • Species:Human
Cat. No. Size Price

YK070 96 wells (1 kit) $779,5
PubMed Product Details
Technical Data

Cat # changed from RSCYK070R to YK070


Competitive ELISA


This EIA kit is used for qu antitative determination of CgA LI in human plasma and saliva samples . The kit is characterized for sensitive quantification, high specificity and no influence with other components in samples. CgA standard is highly purified synthetic product (purity over 98%) and the content indicated is the absolute weight of the standard. N α biotinylglycylglycyl human CgA(344 374)is used as labeled antigen which is proved stability.


Saliva, Plasma

Sample Requirements

25 µl


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.14–33.33 pmol/ml

Intra-assay (Within-Run)

Plasma sample: 10.13 - 13.26 %
Saliva sample: 8.15-12.84 %

Inter-assay (Run-to-Run)

Plasma sample: 11.57-15.33 %
Saliva sample: 12.42 - 14.22 %

Spiking Recovery

Plasma sample: 126.02 %
Saliva sample: 96.6 %



  • RUO
  • The assay kit can measure CgA in the range of 0.14 - 33.33 pmol/mL
  • The assay is completed within 16 20 hr. + 2.5 hr
  • With one assay kit, 41 samples can be measured in duplicate
  • Intraassay CV (%) Plasma 10.13-13.26, saliva 8.15-12.84
  • Interassay CV (%) Plasma 11.57-15.33, saliva 12.42-14.22

Research topic



Chromogranin A (CgA) is an acidic secretory protein consisting of 439 amino acids in human. The protein is found in a wide variety of hormone and neurotransmitter storage vesicles, and it is known be co stored and co released with catecholamines from adrenal medulla and sympathet ic neuronal vesicles during exocytosis. O Conner and Bernstein have first reported radioimmunological measurement of CgA in human plasma under conditions of physiologic, pharmacologic and pathologic alteration of sympathoadrenal function. Accumulated data, thereafter, have confirmed high concentrations of plasma or serum CgA measured by radioimmunoassay (CgA like immunoreactivity: CgA LI) in patients with neuroendocrine and endocrine tumors, especially in those with pheochromocytoma, anterior pituitary tumo rs and rectal an d prostatic carcinoma. On the other hand, Nakane et al. recently discovered that CgA LI exists in saliva, the concentration of which elevates rapidly under psychosomatic stress even prior to the elevation of salivary cortisol level. Subsequ ently, Kanno et al. presented an evidence for autonomic control of submandibular CgA LI secretion in the anaesthetized rat. Most of the reported measurement of CgA by radioimmunoassay utilized native CgA antigens (full or partial length) and antibodies against the native proteins. On the other hand, Yanaihara et al. provided a novel radioimmunoassay system for estimation of Cg A LI level in human plasma with use of synthetic human CgA ( 344 374) and antibody raised against the synthetic peptide. Nakane et al. also used the assay system in their work on human salivary CgA as mentioned above. CgA molecules contain 9 10 sites of bas ic amino acid pairs (Arg Arg, Lys Arg, etc. etc.), which are generally accepted as the proteolytic processing sites. In fact, the sequences corresponding to CgA derived peptides having some biological activities, such as  granin, pancreastatin and parastatin, in CgA molecules are all preceded and followed by basic amino acid pairs. However, it is also known that in the adrenal medulla which is the major site of CgA production, CgA is found to exist predominantly in large molecular forms, supporting the least processing of CgA in adrenal chromaffin cells. In addition, it was shown that there is no rapid degradation of the protein within the bl ood stream. On the basis of these findings, Yanaihara Institute Inc. succeeded in developing, for the first time, a specific, sensitive, stable and easy manipulative enzyme immunoassay (EIA) system for measurement of human CgA LI using anti synthetic human CgA ( 344 374) antibody, synthetic human CgA ( 344 374) as standard antigen and N α biotinylglycylglycyl human CgA ( 344 374) as labeled antigen. The assay kit now available from the Institute can be used for measurement of CgA LI in human biologi cal fluid such as plasma and saliva.

Product References (7)


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  • Konecki DS, Benedum UM, Gerdes HH, Huttner WB. The primary structure of human chromogranin A and pancreastatin. J Biol Chem. 1987 Dec 15;262(35):17026-30. PubMed PMID: 2445752. See more on PubMed
  • Fischer-Colbrie R, Lassmann H, Hagn C, Winkler H. Immunological studies on the distribution of chromogranin A and B in endocrine and nervous tissues. Neuroscience. 1985 Nov;16(3):547-55. doi: 10.1016/0306-4522(85)90191-5. PubMed PMID: 3912673. See more on PubMed
  • O'Connor DT. Chromogranin: widespread immunoreactivity in polypeptide hormone producing tissues and in serum. Regul Pept. 1983 Jul;6(3):263-80. doi: 10.1016/0167-0115(83)90145-3. PubMed PMID: 6622754. See more on PubMed
  • Blaschko H, Comline RS, Schneider FH, Silver M, Smith AD. Secretion of a chromaffin granule protein, chromogranin, from the adrenal gland after splanchnic stimulation. Nature. 1967 Jul 1;215(5096):58-9. doi: 10.1038/215058a0. PubMed PMID: 6053402. See more on PubMed
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