Total 329 AA, UniProt P29279 (Gln27-Ala349). MW: 36 kDa (calculated). C-terminal 6×His tag. Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: >90%
12% SDS-PAGE separation of Human CTGF HEK293:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5μg / lane
3. non-reduced and non-heated sample, 2.5μg / lane
< 0.1 EU/μg
Sterile filtered (0.2 μm) and frozen from 0.10 – 0.30mg/mL solution in 0.1M citrate buffer pH 4,7 containing 20% (w/v) glycerol.
Defrost at ambient temperature
Western blotting, ELISA, Cell culture and/or animal studies
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles. The thawed protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.