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Distributed product

CORTISOL ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Species:Multispecies
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Cat. No. Size Price


New DKO001 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA

Applications

Serum, Plasma

Sample Requirements

20 µl/well

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 10 - 50 - 150 - 500 ng/mL

Limit of Detection

2.42 ng/mL

Intra-assay (Within-Run)

n=16
CV≤ 5.1%

Inter-assay (Run-to-Run)

n=10
CV≤ 11.0%

Spiking Recovery

97.2 - 106.0%

Dilution Linearity

101.5 - 110.9%

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours

The kit measures cortisol

Assay format is 96 wells

Quality Control is ready to use

Calibrators are ready to use

For routine analysis

Research topic

Immune Response, Infection and Inflammation, Reproduction, Steroid hormones

Summary

Cortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid- binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors. The amount of cortisol present in the serum undergoes diurnal variation, with the highest levels present in the early morning, and lower levels in the evening, several hours after the onset of sleep. Highest levels are at about 6-8 a.m. and lowest levels are at about midnight. These normal endogenous functions are the basis for the physiological consequences of chronic stress - prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.

Summary References (7)

References to Cortisol

  • Brock P, Eldred EW, Woiszwillo JE, Doran M, Schoemaker HJ. Direct solid-phase 125I radioimmunoassay of serum cortisol. Clin Chem. 1978 Sep;24 (9):1595-8
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Demers LM, Derck DD. Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine. Clin Biochem. 1977 Jun;10 (3):104-8
  • Morris R. A simple and economical method for the radioimmunoassay of cortisol in serum. Ann Clin Biochem. 1978 May;15 (3):178-83
  • Poland RE, Rubin RT. Saliva cortisol levels following dexamethasone administration in endogenously depressed patients. Life Sci. 1982 Jan 11;30 (2):177-81
  • Silver AC, Landon J, Smith DS, Perry LA. Radioimmunoassay of cortisol in saliva with the "GammaCoat" kit. Clin Chem. 1983 Oct;29 (10):1869-70
  • Vecsei P, Penke B, Katzy R, Baek L. Radioimmunological determination of plasma cortisol. Experientia. 1972 Sep 15;28 (9):1104-5
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