Type
Competitive ELISA, Immobilized antigen
Description
For the quantitative determination of cortisol by enzyme immunoassay in human saliva.
For research use only.
Applications
Saliva
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1–100 ng/ml
Limit of Detection
1.0 ng/ml
Intra-assay (Within-Run)
n = 3; CV = 8.3%
Inter-assay (Run-to-Run)
n = 3; CV = 8.3%
Features
- RUO
- calibration range 1-100 ng/ml
- limit of detection 1 ng/ml
- intra-assay CV = 8.3%
- inter-assay CV = 8.3%
Research topic
Autoimmunity, Immune Response, Infection and Inflammation, Reproduction, Steroid hormones
Summary
Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison's and Cushing's deseases as well as adrenal hyperplasia and carcinoma.
Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1-2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.
Find documents for the lot
Example Instructions for Use (RUO)
Example Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
