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Human Cortisol (Saliva) ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD005R 96 wells (1 kit) $409,44
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the quantitative determination of cortisol by enzyme immunoassay in human saliva. For research use only.



Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1–100 ng/ml

Limit of Detection

1.0 ng/ml

Intra-assay (Within-Run)

n = 3; CV = 8.3%

Inter-assay (Run-to-Run)

n = 3; CV = 8.3%



  • RUO
  • calibration range 1-100 ng/ml
  • limit of detection 1 ng/ml
  • intra-assay CV = 8.3%
  • inter-assay CV = 8.3%

Research topic

Autoimmunity, Immune Response, Infection and Inflammation, Reproduction, Steroid hormones


Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison's and Cushing's deseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1-2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

Product References (1)


  • Reale M, Costantini E, D'Angelo C, Coppeta L, Mangifesta R, Jagarlapoodi S, Di Nicola M, Di Giampaolo L. Network between Cytokines, Cortisol and Occupational Stress in Gas and Oilfield Workers. Int J Mol Sci. 2020 Feb 7;21(3):1118. doi: 10.3390/ijms21031118. PubMed PMID: 32046214. PubMed CentralPMCID: PMC7037782. See more on PubMed
Summary References (7)

References to Cortisol

  • Brock P, Eldred EW, Woiszwillo JE, Doran M, Schoemaker HJ. Direct solid-phase 125I radioimmunoassay of serum cortisol. Clin Chem. 1978 Sep;24 (9):1595-8
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Demers LM, Derck DD. Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine. Clin Biochem. 1977 Jun;10 (3):104-8
  • Morris R. A simple and economical method for the radioimmunoassay of cortisol in serum. Ann Clin Biochem. 1978 May;15 (3):178-83
  • Poland RE, Rubin RT. Saliva cortisol levels following dexamethasone administration in endogenously depressed patients. Life Sci. 1982 Jan 11;30 (2):177-81
  • Silver AC, Landon J, Smith DS, Perry LA. Radioimmunoassay of cortisol in saliva with the "GammaCoat" kit. Clin Chem. 1983 Oct;29 (10):1869-70
  • Vecsei P, Penke B, Katzy R, Baek L. Radioimmunological determination of plasma cortisol. Experientia. 1972 Sep 15;28 (9):1104-5
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