Sandwich ELISA, HRP-labelled antibody
The test is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of degradation products of C-terminal telopeptides of type I collagen in bone cell culture supernatants.
Type I collagen accounts for more than 90% of the organic matrix of bone. During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation. The resorption process can be studied in vitro by culturing bone cells on devitalised slices of bone (catalog # DT-1BON1000-96) or dentin. The CrossLaps® for Culture (CTX-I) ELISA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption occur in the circulation as modified di-peptides. These modified (β-isomerised) and cross-linked di-peptides (Glu-Lys-Ala-His-Asp-β-Gly-Gly-Arg) must be covalently cross-linked through the lysine residue for signal in the CrossLaps® for Culture ELISA. This epitope is present in type I collagen of many species, including human, bovine, elephant and chicken. However, it is not present in rat and mouse.
Cell culture supernatant
50 μL Pre-diluted (1:5)
Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
0.44 nM CrossLaps