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CrossLaps® for Culture ELISA (CTX-I)

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:C-terminal telopeptide I, carboxy-terminal collagen I crosslinks
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Cat. No. Size Price


AC-07F1 96 wells
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Description

The test is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of degradation products of C-terminal telopeptides of type I collagen in bone cell culture supernatants.

Scientific Description:

Type I collagen accounts for more than 90% of the organic matrix of bone. During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation. The resorption process can be studied in vitro by culturing bone cells on devitalised slices of bone (catalog # DT-1BON1000-96) or dentin. The CrossLaps® for Culture (CTX-I) ELISA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption occur in the circulation as modified di-peptides. These modified (β-isomerised) and cross-linked di-peptides (Glu-Lys-Ala-His-Asp-β-Gly-Gly-Arg) must be covalently cross-linked through the lysine residue for signal in the CrossLaps® for Culture ELISA. This epitope is present in type I collagen of many species, including human, bovine, elephant and chicken. However, it is not present in rat and mouse.

Applications

Cell culture supernatant

Sample Requirements

50 μL Pre-diluted (1:5)

Storage/Expiration

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0-112.7 nm

Limit of Detection

0.44 nM CrossLaps

Summary

Features

  • Screening of Anti-Resorptive Drugs
  • High throughput screening of osteoclastic resorptive activity
  • Multiple testing of single osteoclast cultures over time
  • Objective assessment of in vitro bone resorption
  • High correlation to pit area
  • Dynamic monitoring of bone cultures
  • Improved lab productivity due to multi-well screening formats
  • Easy to perform – one step incubation

Research topic

Bone and cartilage metabolism

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